Properties of a Fetal Multipotent Neural Stem Cell (NEP Cell)

Developmental Biology

Jingli Cai¹  Kurt H. Albertine¹  Gerald J. Spangrude¹  Takumi Mirua¹  Mary T. Lucero¹  Yuanyuan Wu¹  Jeanne L. Pierce¹  Mahendra S. Rao¹ 

[1] Laboratory of Neurosciences, National Institute on Aging, Baltimore, Maryland, 21224

关键词: NSC;    NEP;    NRP;    GRP;    differentiation;    precursor;   

DOI  :  10.1006/dbio.2002.0828

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Multipotentneuralstemcells(NSCs)presentinthedevelopingneuraltube(E10.5,neuroepithelialcells;NEP)wereexaminedfortheexpressionofcandidatestemcellmarkers,andtheexpressionofthesemarkerswascomparedwithlaterappearingprecursorcells(E14.5)thatcanbedistinguishedbytheexpressionofembryonicneuralcelladhesionmolecule(E-NCAM)andA2B5.NEPcellspossessgapjunctions,expressconnexins,andappeartolacklongcilia.Mostcandidatemarkers,includingNestin,Presenilin,Notch,andNumb,wereexpressedbybothNEPcellsaswellasothercellpopulations.Fibroblastgrowthfactorreceptor4(FGFR4),Frizzled9(Fz9),andSRYbox-containinggene2(Sox2)asassessedbyimmunocytochemistryandinsituhybridizationaremarkersthatappeartodistinguishNSCsfromotherprecursorcells.NeitherHoechst33342norrhodamine-123staining,telomerase(Tert)expression,telomeraseactivity,orbreakpointclusterregionprotein1(Bcrp1)transporterexpressioncouldbeusedtodistinguishNEPstemcellsfromotherdividingcells.NEPcells,however,lackedexpressionofseverallineagemarkersthatareexpressedbylaterappearingcells.TheseincludedabsenceofexpressionofCD44,E-NCAM,A2B5,epidermalgrowthfactorreceptor(EGFR),andplatelet-derivedgrowthfactorreceptor-alpha(PDGFRα),suggestingthatnegativeselectionusingcellsurfaceepitopescouldbeusedtoisolatestemcellpopulationsfrommixedculturesofcells.UsingmixedculturesofcellsisolatedfromE14.5stageembryos,weshowthatNEPcellscanbeenrichedbydepletingdifferentiatingcellsthatexpressE-NCAMorA2B5immunoreactivity.Overall,ourresultsshowthataspectrumofmarkersusedincombinationcanreliablydistinguishmultipotentNSCsfromotherprecursorcellsaswellasdifferentiatedcellspresentintheCNS.

【 授权许可】

Unknown