Constitutive Expression of Preproendothelin in the Cardiac Neural Crest Selectively Promotes Expansion of the Adventitia of the Great Vessels in Vivo

Developmental Biology

Takashi Mikawa¹  Victoria L.T. Ballard¹ 

[1] Department of Cell Biology, Cornell University Medical College, 1300 York Avenue, New York, New York, 10021

关键词: cardiac neural crest;    arch artery;    great vessels;    endothelin;    retrovirus;    chicken embryo;    heart development;   

DOI  :  10.1006/dbio.2002.0818

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Cardiacneuralcrestcellsareessentialfornormaldevelopmentofthegreatvesselsandtheheart,givingrisetoarangeofcelltypes,includingbothneuronalandnon-neuronaladventitialcellsandsmoothmuscle.Endothelin(ET)signalingplaysanimportantroleinthedevelopmentofcardiacneuralcrestcelllineages,yettheunderlyingmechanismsthatacttocontroltheirmigration,differentiation,andproliferationremainlargelyunclear.Weexaminedtheexpressionpatternsofthereceptor,ETA,andtheET-specificconvertingenzyme,ECE-1,inthepharyngealarchesandgreatvesselsofthedevelopingchickembryo.Insituhybridizationanalysisrevealedthat,whileETAisexpressedinthepharyngealarchmesenchyme,populatedbycardiacneuralcrestcells,ECE-1expressionislocalizedtotheoutermostectodermalcellsofthearchesandthentotheinnermostendothelialcellsofthegreatvessels.ThisdynamicpatternofexpressionsuggeststhatonlyasubpopulationofneuralcrestcellsintheseregionsisresponsivetoETsignalingatparticulardevelopmentaltimepoints.Totestthis,retroviralgenedeliverywasusedtoconstitutivelyexpresspreproET-1,aprecursorofmatureET-1ligand,inthecardiacneuralcrest.Thisresultedinaselectiveexpansionoftheoutermost,adventitialcellpopulationinthegreatvessels.Incontrast,neitherdifferentiationnorproliferationofneuralcrest-derivedsmoothmusclecellswassignificantlyaffected.TheseresultssuggestthatconstitutiveexpressionofexogenouspreproET-1inthecardiacneuralcrestresultsinexpansionrestrictedtoanadventitialcellpopulationofthedevelopinggreatvessels.

【 授权许可】

Unknown