Extracellular Matrix Remodeling and Metalloproteinase Involvement during Intestine Regeneration in the Sea Cucumber Holothuria glaberrima

Developmental Biology

José L. Quiñones¹  José E. García-Arrarás¹  Rey Rosa¹  Dorcas L. Ruiz¹ 

[1] Department of Biology, University of Puerto Rico, Rı́o Piedras, Puerto Rico, 00931

关键词: echinoderm;    extracellular matrix remodeling;    ECM;    epithelial tubular outgrowth;    fibrosis;    holothuroid;    intestine regeneration;    metalloproteases;    metalloprotease inhibitors;    morphogenesis;    organogenesis;    1;    10-phenanthroline;    zymograph;   

DOI  :  10.1006/dbio.2002.0778

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theseacucumber,Holothuriaglaberrima,hasthecapacitytoregenerateitsinternalorgans.Intestinalregenerationisaccomplishedbythethickeningofthemesentericborderandtheinvasionofthisthickeningbymucosalepitheliumfromtheesophagusandthecloaca.Extracellularmatrix(ECM)remodelinghasbeenassociatedwithmorphogeneticeventsduringembryonicdevelopmentandregeneration.WehaveusedimmunohistochemicaltechniquesagainstECMcomponentstoshowthatdifferentialchangesoccurintheECMduringearlyregeneration.Labelingoffibrouscollagenouscomponentsandmuscle-relatedlaminindisappearfromtheregeneratingintestineandmesentery,whilefibronectinlabelingand4G7(anechinodermECMcomponent)arecontinuouslypresent.Westernblotsconfirmadecreaseinfibrouscollagencontentduringthefirst2weeksofregeneration.Wehavealsoidentifiedfive1,10-phenanthroline-sensitivebandsincollagengelatinzymographs.Thegelatinolyticactivitiesofthesebandsareenhancedduringearlystagesofregeneration,suggestingthatthemetalloproteaseactivityisassociatedwithECMremodeling.InhibitionofMMPsinvivowith1,10-phenanthroline,p-aminobenzoyl-Gly-Pro-D-Leu-D-AlahydroxamateorN-CBZ-Pro-Leu-GlyhydroxamateproducesareversibleinhibitionofintestinalregenerationandECMremodeling.OurresultsshowthatsignificantchangesinECMcontentoccurduringintestineregenerationintheseacucumberandthattheonsetofthesechangesiscorrelatedtotheproteolyticactivitiesofMMPs.

【 授权许可】

Unknown