Requirement for Both EDEN and AUUUA Motifs in Translational Arrest of Mos mRNA upon Fertilization of Xenopus Eggs

Developmental Biology

Shuichi Ueno¹  Noriyuki Sagata¹ 

[1] Department of Biology, Graduate School of Sciences, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan

关键词: AUUUA motif;    EDEN;    Eg2 mRNA;    Mos mRNA;    translation;    Xenopus egg;   

DOI  :  10.1006/dbio.2002.0787

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

TranslationalarrestofmaternalMosmRNAuponfertilizationofXenopuseggsisaprerequisitefortheinitiationofembryonicdivisions.Recentstudiessuggestthatanembryodeadenylationelement(EDEN)presentinthe3′untranslatedregion(3′UTR)issufficientfordeadenylation(and,hence,probablyfortranslationalarrest)ofMosmRNAafterfertilization.BydirectlymonitoringtranslationofnumerousMosmRNAconstructsinXenopuseggs,however,weshowherethattheEDENisnecessarybutnotsufficientfortranslationalarrestofMosmRNA.WedemonstratethattwoAUUUAmotifs,eachlocatedsolitarilyanddistantlyfromtheEDEN,arealsorequiredforthetranslationalarrestofMosmRNAafterfertilization.Significantly,translationalarrestofEg2mRNA,anotherEDEN-containingmaternalmRNA,alsorequiresasingleAUUUAmotiflocatedfarfromtheEDEN.Analysisofthepoly(A)tailsofvariousMosmRNAconstructsindicatesthattheEDENaloneconfersonlypartialdeadenylationonMosmRNA,andthattheAUUUAmotifsacttoenhanceEDEN-directeddeadenylationinaposition-dependentmanner.Finally,introductionofanexcessoftheEDEN,butnottheAUUUAmotifs,intoeggscanrestoretranslationofendogenousMosmRNA.TheseresultssuggestthattheEDEN,onlytogetherwithappropriatelypositionedAUUUAmotifsandatrans-actingfactor(s),canefficientlydeadenylateandhencetranslationallyarrestMos(aswellasEg2)mRNAafterfertilization.

【 授权许可】

Unknown