Sonic Hedgehog Activates Mesenchymal Gli1 Expression during Prostate Ductal Bud Formation

Developmental Biology

Philip Iannaccone¹  Winnie S Catbagan¹  David Walterhouse¹  William Gaffield¹  Marilyn L.G Lamm¹  Wade Bushman¹  Robert J Laciak¹  Daniel H Barnett¹  Christy M Hebner¹ 

[1] Department of Urology, Northwestern University Medical School, Tarry Building 11-715, 303 East Chicago Avenue, Chicago, Illinois, 60611

关键词: Sonic hedgehog;    Gli;    Ptc1;    prostate development;    urogenital sinus;    ductal budding;    cyclopamine;   

DOI  :  10.1006/dbio.2002.0774

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Ductalbuddinginthedevelopingprostateisatestosterone-dependenteventthatinvolvessignalingbetweentheurogenitalsinusepithelium(UGE)andurogenitalsinusmesenchyme(UGM).WeshowherethatductalbudformationisassociatedwithfocusedexpressionofSonichedgehog(Shh)intheepitheliumofnascentprostatebudsandinthegrowingtipsofelongatingprostateducts.ThispatternoflocalizedShhexpressionoccursinresponsetotestosteronestimulation.ThegenefortheShhreceptor,Ptc1,isexpressedintheUGM,asarethemembersoftheGligenefamilyoftranscriptionalregulators(Gli1,Gli2,andGli3).ExpressionofPtc1,Gli1,andGli2islocalizedprimarilytomesenchymesurroundingprostatebuds,whereasGli3isexpresseddiffuselythroughouttheUGM.AstrongdependenceofGli1(andPtc1)expressiononShhsignalingisdemonstratedbyinductionofexpressioninboththeintacturogenitalsinusandtheisolatedUGMbyexogenousSHHpeptide.AsimilardependenceofGli2andGli3expressiononShhisnotobserved.Nonetheless,thechemicalinhibitorofShhsignaling,cyclopamine,producedagradedinhibitionofGligeneexpression(Gli1>Gli2>Gli3)inurogenitalsinusexplantsthatwasparalleledbyasevereinhibitionofductalbudding.

【 授权许可】

Unknown