The Pattern of Neurovascular Development in the Forelimb of the Quail Embryo

Developmental Biology

Donald F Newgreen¹  Damien Bates¹  G.Ian Taylor¹ 

[1] Embryology Laboratory, MCRI, Royal Children's Hospital, Flemington Road, Parkville, Victoria, 3052, Australia

关键词: neurovascular;    nerves;    blood vessels;    endothelium;    patterning;    quail;    forelimb;    median artery;   

DOI  :  10.1006/dbio.2002.0771

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Peripheralnerveandvascularpatternsarecongruentintheadultvertebrate,butthishasbeendisputedinvertebrateembryos.Themostdetailedofthesestudieshaveusedtheavianforelimbasamodelsystem,yetneurovascularanatomicalrelationshipsandcriticalvascularremodelingeventsremaininadequatelycharacterizedinthismodel.Toaddressthis,wehaveusedacombinationofintravascularmarkerinjection,multilabelfluorescentstereomicroscopy,andconfocalmicroscopytoanalyzethespatiotemporalrelationshipsbetweenperipheralnervesandbloodvesselsintheforelimbof818quailembryosfromE2(HH13)toE15(HH41).Wefindthattheneurovascularanatomicalrelationshipsestablishedduringdevelopmentarehighlystereotypicandcongruent.Bloodvesselstypicallyarisebeforetheircorrespondingnerves,butthereareseveralcriticalexceptionstothisrule.Thevascularpatternisextensivelyremodeledfromtheearlieststageexamined(E2;HH13),whereastheperipheralnerves,thefirstofwhichentertheforelimbatE3.5–E4(HH21–HH24),haveaprogressivelyunfoldingpatternthat,onceformed,remainsessentiallyunchanged.TheadultneurovascularpatternisnotestablisheduntilE8(HH34).Peripheralnervesarealwaysfoundtotrackcloseandparalleltothevasculature.Astheytrackdistally,peripheralnervesalwayslieonthesideofthevasculatureawayfromthecenteroftheforelimb.Neurovascularpatternshaveahierarchyofcongruencethatishighestinthedorsoventralplane,followedbytheanteroposterior,andlastlytheproximodistalplanes.

【 授权许可】

Unknown