Developmental Biology | |
The Pattern of Neurovascular Development in the Forelimb of the Quail Embryo | |
Donald F Newgreen1  Damien Bates1  G.Ian Taylor1  | |
[1] Embryology Laboratory, MCRI, Royal Children's Hospital, Flemington Road, Parkville, Victoria, 3052, Australia | |
关键词: neurovascular; nerves; blood vessels; endothelium; patterning; quail; forelimb; median artery; | |
DOI : 10.1006/dbio.2002.0771 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
Peripheralnerveandvascularpatternsarecongruentintheadultvertebrate,butthishasbeendisputedinvertebrateembryos.Themostdetailedofthesestudieshaveusedtheavianforelimbasamodelsystem,yetneurovascularanatomicalrelationshipsandcriticalvascularremodelingeventsremaininadequatelycharacterizedinthismodel.Toaddressthis,wehaveusedacombinationofintravascularmarkerinjection,multilabelfluorescentstereomicroscopy,andconfocalmicroscopytoanalyzethespatiotemporalrelationshipsbetweenperipheralnervesandbloodvesselsintheforelimbof818quailembryosfromE2(HH13)toE15(HH41).Wefindthattheneurovascularanatomicalrelationshipsestablishedduringdevelopmentarehighlystereotypicandcongruent.Bloodvesselstypicallyarisebeforetheircorrespondingnerves,butthereareseveralcriticalexceptionstothisrule.Thevascularpatternisextensivelyremodeledfromtheearlieststageexamined(E2;HH13),whereastheperipheralnerves,thefirstofwhichentertheforelimbatE3.5–E4(HH21–HH24),haveaprogressivelyunfoldingpatternthat,onceformed,remainsessentiallyunchanged.TheadultneurovascularpatternisnotestablisheduntilE8(HH34).Peripheralnervesarealwaysfoundtotrackcloseandparalleltothevasculature.Astheytrackdistally,peripheralnervesalwayslieonthesideofthevasculatureawayfromthecenteroftheforelimb.Neurovascularpatternshaveahierarchyofcongruencethatishighestinthedorsoventralplane,followedbytheanteroposterior,andlastlytheproximodistalplanes.
【 授权许可】
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【 预 览 】
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