N-Twist, an Evolutionarily Conserved bHLH Protein Expressed in the Developing CNS, Functions as a Transcriptional Inhibitor

Developmental Biology

Kathleen K. Kelly¹  Brian J. North¹  Brian L. Black¹  Evdokia Dodou¹  Stephanie B. Greene¹  Joshua P. Anderson¹  Richard M. Cripps¹  Michael P. Verzi¹ 

[1] Cardiovascular Research Institute, University of California, San Francisco, California, 94143-0130

关键词: N-twist;    basic-helix–loop–helix;    bHLH;    achaete-scute;    neurogenesis;    MASH1;    inhibitor;    transcription;    brain;    neural tube;    mouse;    Drosophila;   

DOI  :  10.1006/dbio.2002.0753

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Membersofthebasichelix–loop–helix(bHLH)transcriptionfactorfamilyplayanessentialroleinmultipledevelopmentalprocesses.Duringneurogenesis,positiveandnegativeregulationbybHLHproteinsisessentialforproperdevelopment.HerewereporttheidentificationandinitialcharacterizationofthebHLHgene,Neuronaltwist(N-twist),namedforitsneuralexpressionpatternandhighsequencehomologyandphysicallinkagetothemesodermalinhibitor,M-twist.N-twistisexpressedinthedevelopingmousecentralnervoussysteminthemidbrain,hindbrain,andneuraltube.Thisneuralexpressionisconservedininvertebrates,asexpressionoftheDrosophilaorthologofN-twistisalsorestrictedtothecentralnervoussystem.LikeotherbHLHfamilymembers,N-TwistheterodimerizeswithEproteinandbindsDNAataconsensusbHLH-bindingsite,theEbox.WeshowthatN-TwistinhibitsMASH1-dependenttranscriptionalactivationbysequesteringEproteininadominantnegativefashion.Thus,thesestudiessupportthenotionthatN-Twistrepresentsanovelnegativeregulatorofneurogenesis.

【 授权许可】

Unknown