Activated MAPK/ERK Kinase (MEK-1) Induces Transdifferentiation of Pigmented Epithelium into Neural Retina

Developmental Biology

Bertrand Néron¹  Nathalie Planque¹  Simon Saule¹  Alain Eychène¹  Anne Galy¹ 

[1] CNRS UMR 146, Institut Curie, Centre Universitaire, Laboratoire 110, 91405, Orsay Cedex, France

关键词: retina;    MAPK;    Ras;    Raf;    pigment;    microphthalmia;    FGF;    signaling;    embryo;   

DOI  :  10.1006/dbio.2002.0736

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringvertebrateeyedevelopment,theopticvesicleoriginatingfromtheneuroectodermispartitionedintoadomainthatwillgiverisetotheneuralretina(NR)andanotherthatwillgiverisetotheretinalpigmentedepithelium(RPE).PreviousstudieshaveshownthatectopicexpressionofFGFsintheRPEinducesRPE-to-NRtransdifferentiation.Similarly,anaturallyoccurringmutationofthetranscriptionfactorMitfinmouseresultedintheformationofasecondneuralretinainplaceofthedorsalRPE,buttheputativesignalingpathwaylinkingFGFtoMitfregulationispresentlyunknown.Inculturesofneuralcrest-derivedmelanocytes,theMAPKpathwaywasrecentlyshowntotargettheMitftranscriptionfactorforubiquitin-dependentproteolysis,resultinginarapiddegradationanddownregulation.Inthepresentstudy,weshowthatectopicexpressionofaconstitutivelyactivatedalleleofMEK-1,theimmediateupstreamactivatoroftheMAPKERK,inchickenembryonicretinainovo,inducestransdifferentiationoftheRPEintoaneural-likeepitheliumthatiscorrelatedwithadownregulationofMitfexpressioninthepresumptiveRPE.

【 授权许可】

Unknown