An Epithelial Precursor Is Regulated by the Ureteric Bud and by the Renal Stroma

Developmental Biology

Thaddeus Novak¹  Randy Levinson¹  Eseng Lai¹  Jun Yang¹  Alexander Blum¹  Jonathan Barasch¹ 

[1] College of Physicians and Surgeons of Columbia University, New York, New York, 10032

关键词: epithelia;    progenitor;    mesenchyme;    induction;    kidney;    stroma;   

DOI  :  10.1006/dbio.2002.0646

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Kidneyepitheliadevelopfromthemetanephricmesenchymeafterreceivinginductivesignalsfromtheuretericbudandfromtherenalstroma.However,itisnotclearhowthesesignalsinducethedifferenttypesofepitheliathatmakeupthenephron.Toinvestigateinductivesignaling,wehaveisolatedclustersofepithelialprogenitorsfromthemetanephricmesenchyme,therebyseparatingthemfromtherenalstroma.WhentheisolatedprogenitorsweretreatedwiththeuretericbudfactorLIF,theyexpressedepithelialproteins(ZO-1,E-cadherin,lamininα5)andproducednephrons(36glomeruliwith58tubules),indicatingthattheyarethetargetofinductivesignalingfromtheuretericbud,andthatrenalstromaisnotabsolutelyrequiredforepithelialdevelopmentinvitro.Infact,stroma-depletedepithelialprogenitorsproducedsevenfoldmoreglomerulithandidintactmetanephricmesenchyme(5glomeruli,127tubules).Conversely,whenepithelialprogenitorsweretreatedwithbothLIFandproteinssecretedfromarenalstromalcellline,glomerulogenesiswasabolishedbuttubularepitheliawereexpanded(0glomeruli,47tubules).Hence,byisolatingepithelialprogenitorsfromthemetanephricmesenchyme,weshowthattheyaretargetedbyfactorsfromtheuretericbudandfromtherenalstroma,andthatepithelialdiversificationisstimulatedbytheuretericbudandlimitedbyrenalstroma.

【 授权许可】

Unknown