Joint Development in the Drosophila Leg: Cell Movements and Cell Populations

Developmental Biology

Michael Akam¹  Christen Mirth¹ 

[1] Department of Zoology, University Museum of Zoology, Downing Street, Cambridge, CB2 3EJ, United Kingdom

关键词: arthropod limb;    leg imaginal discs;    metamorphosis;    morphogenesis;    cell shape;    joint territories;    tibia;    tarsus;    SEM;    GAL4 enhancer trap;    plasmid rescue;   

DOI  :  10.1006/dbio.2002.0593

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Flexiblejointsseparatetherigidsectionsoftheinsectleg,allowingthemtomove.InDrosophila,theinitialpatterningofthesejointsisapparentinthelarvalimaginaldiscsfromwhichtheadultlegswilldevelop.Here,wedescribethelaterpatterningandmorphogenesisofthejoints,whichoccursafterpupariation(AP).Inthetibial/tarsaljoint,theapodemeinsertionsiteprovidesafixedmarkerfortheboundarybetweenproximalanddistaljointterritories(theP/Dboundary).Cellsoneithersideofthisboundarybehavedifferentlyduringmorphogenesis.Morphogenesisbeginswiththeapicalconstrictionofdistaljointcells,about24hAP.Distalcellsthenbecomecolumnar,causingdistaltissuenearesttheP/Dboundarytofoldintotheleg.Inthelaststageofjointmorphogenesis,theproximaljointcellsclosesttotheP/Dboundaryalignandelongatetoforma“palisade”(arowofcolumnarcells)overthedistaljointcells.Theproximalanddistaljointterritoriesarecharacterisedbythedifferentialorganisationofcytoskeletalandextracellularmatrixproteins,andbythedifferentialexpressionofenhancertraplinesandothergenemarkers.Thesemarkersalsodefineanumberofmorelocalisedterritorieswithinthepupaljoint.

【 授权许可】

Unknown