A Germline-Specific Splicing Generates an Extended Ovo Protein Isoform Required for Drosophila Oogenesis

Developmental Biology

Alain Vincent¹  Maryvonne Mével-Ninio¹  François Payre¹  Cathy Salles¹ 

[1] Centre de Biologie du Développement, UMR CNRS 5547, Toulouse, France, 31062 cedex 4

关键词: ovo/shavenbaby;    oogenesis;    germline;    epidermis;    transcription factors;    alternative splicing;    retrotransposon;   

DOI  :  10.1006/dbio.2002.0659

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Mostregulatorygenesareemployedmultipletimestocontroldifferentprocessesduringdevelopment.TheDrosophilaOvo/Shavenbaby(Svb)transcriptionfactorisrequiredbothforgermlineandepidermaldifferentiation,tworolesalsofoundforitsorthologm-ovo1inmice.InDrosophila,thesetwodistinctfunctionsarecontributedbyseparatecontrolregionsdirectingtheexpressionofOvo/Svbinthegermline(ovo)andsoma(svb),respectively.WereportherethatalternativesplicingrepresentsanadditionalleveloftheregulationofOvo/Svbfunctionalspecificity.CharacterizationoftheovoD1rv23mutationrevealedthattheintragenicinsertionofanovelretrotransposon,romano,inactivatesovowithoutalteringsvb.Weprovideevidencethatthisinsertiondisruptsagermline-specificalternativeexon,exon2b,whichencodesa178-amino-acidinternalextension(2B).Whilebothisoforms,Ovo+2BandOvo−2B,accumulateduringoogenesis,onlyOvo+2Bisabletofulfillgerminalovofunctions.Ovo−2Bisunable,evenwhenoverexpressed,tofullyrescueoogenicdefectsresultingfromtheabsenceofwildtypeovoproduct.Bycontrast,eitherOvo+2BorOvo−2BgermlineproteincansubstituteforSvbintheepidermis.Ourresultsemphasizethespecificfeaturesofsplicinginthegermline,andrevealitsfunctionalimportanceforthecontrolofovo/svb-dependentovarianandepidermaldifferentiation.

【 授权许可】

Unknown