Disruption of Testis Cords by Cyclopamine or Forskolin Reveals Independent Cellular Pathways in Testis Organogenesis

Developmental Biology

Humphrey Hung-Chang Yao¹  Blanche Capel¹ 

[1] Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, 27710

关键词: desert hedgehog;    Patched1;    Sertoli;    Leydig;    germ cells;    meiosis;    PKA;   

DOI  :  10.1006/dbio.2002.0663

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

MoststudiestodateindicatethattheformationoftestiscordsiscriticalforproperSertolicelldifferentiation,inhibitionofgermcellmeiosis,andregulationofLeydigcelldifferentiation.However,theconnectionsbetweentheseeventsarepoorlyunderstood.Theobjectiveofthisstudywastodissectthemolecularandcellularrelationshipsbetweentheseeventsintestisformation.Wetookadvantageofthedifferenteffectsoftwohedgehogsignalinginhibitors,cyclopamineandforskolin,ongonadexplantcultures.BothhedgehoginhibitorsphenocopiedthedisruptiveeffectofDhh−/−onformationoftestiscordswithoutinfluencingSertolicelldifferentiation.However,theyexhibiteddifferenteffectsonothercellulareventsduringtestisdevelopment.TreatmentwithcyclopaminedidnotaffectinhibitionofgermcellmeiosisandmesonephriccellmigrationbutcauseddefectsinLeydigcelldifferentiation.Incontrast,forskolintreatmentinducedgermcellmeiosis,inhibitedmesonephriccellmigration,andhadnoeffectonLeydigcelldifferentiation.Bycarefullycontrastingthedifferenteffectsofthesetwohedgehoginhibitors,wedemonstratethat,althoughformationoftestiscordsanddevelopmentofothercelltypesnormallytakeplaceinatightlyregulatedsequence,eachoftheseeventscanoccurindependentoftheothers.

【 授权许可】

Unknown