Indian Hedgehog as a Progesterone-Responsive Factor Mediating Epithelial–Mesenchymal Interactions in the Mouse Uterus

Developmental Biology

Sudhansa K. Dey¹  Sanjoy K. Das¹  Brigid L.M. Hogan¹  Xuemei Zhao¹  Hiromichi Matsumoto¹ 

[1] Departments of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas, 66160-7336

关键词: Indian hedgehog;    patched;    gli;    noggin;    uterus;    progesterone;    pregnancy;   

DOI  :  10.1006/dbio.2002.0645

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Genesencodingcomponentsofthehedgehogsignalingpathwayaredynamicallyexpressedinthemouseuteruspreparingforimplantation.Indianhedgehog(Ihh),patched(Ptc),andGli3areexpressedatlowlevelsintheendometrialepitheliumonday1ofpregnancy.TranscriptionofIhhincreasesdramaticallyintheluminalepitheliumandglandsfromday3,reachingveryhighlevelsonday4.Overthesameperiod,Ptc,Gli1,Gli2,andnogginarestronglyupregulatedintheunderlyingmesenchymalstroma.TranscriptionofIhhinovariectomizedmiceisinducedbyprogesteronebutnotbyestrogen.LowerinductionofIhh,Ptc,andHoxa10isseeninresponsetoprogesteroneintheuteriofPgr−/−mutantmicelackingprogesteronenuclearsteroidreceptor.ThisfindingsuggeststhatthehormonemayregulateIhhthroughbothnuclearreceptor-dependentand-independentpathways.Wedescribeamethodforculturinguterineexplantsintheabsenceofepithelium.Undertheseconditions,recombinantN-SHHproteinpromotestheproliferationofmesenchymecellsandtheexpressionofnoggin.WeproposethatIHHmadebytheepitheliumnormallyfunctionsasaparacrinegrowthfactorforstromalcellsduringtheearlystagesofpregnancy.

【 授权许可】

Unknown