Analysis of Spatial and Temporal Gene Expression Patterns in Blastula and Gastrula Stage Chick Embryos

Developmental Biology

Andrew Lumsden¹  Susan C. Chapman¹  Frank R. Schubert¹  Gary C. Schoenwolf¹ 

[1]MRC Centre for Developmental Neurobiology, Kings College London, New Hunts House, Guy's Hospital, London, SE1 1UL, United Kingdom

关键词: epiblast;    forebrain;    head;    hypoblast;    induction;    organiser;    patterning;    trunk;    tail;    endoderm;   

DOI  :  10.1006/dbio.2002.0641

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Studiesonthegeneticbasisofrostral–caudalspecification,neuralinduction,andheaddevelopmentrequireknowledgeoftherelevantgeneexpressionpatterns.Gapsinourunderstandingofgeneexpressionhaveledustoexaminethedetailedspatiotemporalexpressionpatternsof19genesimplicatedinearlydevelopment,tolearnmoreabouttheirpotentialroleinspecifyingandpatterningearlydevelopmentalprocessesleadingtoheadformation.Here,wereporttheexpressionpatternsofthesemarkersinblastula-andgastrula-stagechickembryos,usingwhole-mountinsituhybridisation.Nodal,Fgf8,Bmp7,Chordin,Lim1,Hnf3β,Otx2,Goosecoid,Cerberus,Hex,Dickkopf1,andCrescentareallalreadyexpressedbythetimetheeggislaid.Whentheprimitivestreakhasreacheditsfulllength,alatergroupofgenes,includingGanf,Six3,Bmp2,Bmp4,Noggin,Follistatin,andQin(BF1),beginstobeexpressed.Wereassesscurrentmodelsofearlyrostralpatterningbasedontheanalysisofthesedynamicspatiotemporalexpressionpatterns.

【 授权许可】

Unknown