Sca-1pos Cells in the Mouse Mammary Gland Represent an Enriched Progenitor Cell Population

Developmental Biology

Teresa Venezia¹  Jeffrey M. Rosen¹  Margaret A. Goodell¹  Stacey B. Tepera¹  Bryan E. Welm¹  Timothy A. Graubert¹ 

[1] Program in Cell and Molecular Biology, Department of Pediatrics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas, 77035

关键词: mammary;    stem cells;    BrdU;    Sca-1;    label retention;    progesterone receptor;   

DOI  :  10.1006/dbio.2002.0625

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Mammaryepitheliumcanfunctionallyregenerateupontransplantation.Thisrenewalcapacityhasbeenclassicallyascribedtothefunctionofamultipotentmammaryglandstemcellpopulation,whichhasbeenhypothesizedtobeaprimarytargetintheetiologyofbreastcancer.Severalcomplementaryapproacheswereemployedinthisstudytoidentifyandenrichmammaryepithelialcellsthatretainstemcellcharacteristics.Usinglong-termBrdUlabeling,apopulationoflabelretainingcells(LRCs)thatlackexpressionofdifferentiationmarkershasbeenidentified.LRCsisolatedfrommammaryprimarycultureswereenrichedforstemcellantigen-1(Sca-1)andHoechstdye-effluxing“sidepopulation”properties.Sca-1poscellsinthemammaryglandwerelocalizedtotheluminalepitheliabyusingSca-1+/GFPmice,wereprogesteronereceptor-negative,anddidnotbindpeanutlectin.Finally,theSca-1pospopulationisenrichedforfunctionalstem/progenitorcells,asdemonstratedbyitsincreasedregenerativepotentialcomparedwithSca-1negcellswhentransplantedintotheclearedmammaryfatpadsofhostmice.

【 授权许可】

Unknown