The Major Yolk Protein in Sea Urchins Is a Transferrin-like, Iron Binding Protein

Developmental Biology

Jacqueline M. Brooks¹  Gary M. Wessel¹ 

[1] Department of Molecular and Cell Biology and Biochemistry, Division of Biology and Medicine, Brown University, Providence, Rhode Island, 02912

关键词: oogenesis;    major yolk protein (MYP);    yolk platelet;    vitellogenin;    transferrin;    iron;   

DOI  :  10.1006/dbio.2002.0611

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Themajoryolkprotein(MYP)inseaurchinshashistoricallybeenclassifiedasavitellogeninbasedonitsabundanceintheyolkplatelets.Curiously,itisfoundinbothsexesofseaurchinswhereitispresumedtoplayaphysiologicalroleingametogenesis,embryogenesis,orboth.HerewepresenttheprimarystructureofMYPaspredictedfromcDNAsoftwoseaurchinsspecies,StrongylocentrotuspurpuratusandLytechinusvariegatus.Thesequencefromthesetwospeciesshareidentitytooneanother,butbearnoresemblancetootherknownvitellogenins.Insteadthesequencesharesidentitytomembersofthetransferrinsuperfamilyofproteins.Invitroironbindingassays,includingboth59FeoverlayassaysofMYPenrichedcoelomicfluidandimmunoprecipitationofnativeiron-boundMYPfromcoelomicfluid,supportthisclassification.WesuggestthatoneofMYP'stransferrin-likepropertiesistoshuttleirontodevelopinggermcells.

【 授权许可】

Unknown