Epithelia–Mesenchyme Interaction Plays an Essential Role in Transdifferentiation of Retinal Pigment Epithelium of silver Mutant Quail: Localization of FGF and Related Molecules and Aberrant Migration Pattern of Neural Crest Cells during Eye Rudiment Formation

Developmental Biology

Masasuke Araki¹  Takako Takano¹  Tomoko Kaneko¹  Masaoki Tsudzuki¹  Yoshifumi Nakane¹  Tomoko Uemonsa¹ 

[1] Developmental Neurobiology Laboratory, Department of Biological Sciences, Nara Women's University, Nara, 630-8506, Japan

关键词: retina;    retinal pigment epithelium;    neural crest cell;    laminin;    heparan sulfate;    fibroblast growth factors;    chimera embryo;    transdifferentiation;    silver mutation;    Mitf;   

DOI  :  10.1006/dbio.2002.0591

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Homozygotesofthequailsilvermutation,whichhaveplumagecolorchanges,alsodisplayauniquephenotypeintheeye:duringearlyembryonicdevelopment,theretinalpigmentepithelium(RPE)spontaneouslytransdifferentiatesintoneuralretinaltissue.Mitfisconsideredtobetheresponsiblegeneandtofunctionsimilarlytothemousemicrophthalmiamutation,andtissueinteractionbetweenRPEandsurroundingmesenchymaltissueinorganculturehasbeenshowntobeessentialfortheinitiationofthetransdifferentiationprocessinwhichfibroblastgrowthfactor(FGF)signalingisinvolved.Theimmunohistochemicalresultsofthepresentstudyshowthatlamininandheparansulfateproteoglycan,bothactingascofactorsforFGFbinding,arelocalizedintheareaoftransdifferentiationofsilverembryosmuchmoreabundantlythaninwild-typeembryos.MoreintenseimmunohistochemicalstainingwithFGF-1antibody,butnotwithFGF-2antibody,isalsofoundintheneuralretina,RPE,andchoroidaltissueofsilverembryosthaninwild-typeembryos.HNK-1immunohistochemistryrevealedthatclustersofHNK-1-positivecells(presumptivemigratingneuralcrestcells)arefrequentlylocatedaroundthedevelopingeyesandintheposteriorregionofthesilverembryoniceye.Finally,chick–quailchimericaleyesweremadebygraftingsilverquailopticvesiclestochickenhostembryos:inmostcases,notransdifferentiationoccursinthesilverRPE,butinafewcases,transdifferentiationoccurswheresilverquailcellspredominateinthechoroidtissue.TheseobservationstogetherwithourpreviousinvitrostudyindicatethatthesilvermutationaffectsnotonlyRPEcellsbutalsocephalicneuralcrestcells,whichmigratetotheeyerudiment,andthatthesecrestcellsplayanessentialroleinthetransdifferentiationofRPE,possiblybymodifyingtheFGFsignalingpathway.TheprecisemolecularmechanisminvolvedinRPE–neuralcrestcellinteractionisstillunknown,andthequailsilvermutationisconsideredtobeagoodexperimentalmodelforstudyingtheroleofneuralcrestcellsinvertebrateeyedevelopment.

【 授权许可】

Unknown