Regulation of Synaptotagmin Gene Expression during Ascidian Embryogenesis

Developmental Biology

Haruo Okado¹  Yasushi Okamura¹  Toshiaki Okada¹  You Katsuyama¹  Ling Chen¹  Yukio Ohtsuka¹  Jun Matsumoto¹ 

[1]Molecular Neurobiology Group, Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, Higashi 1-1-1, Tsukuba, 305-8566, Japan

关键词: neuronal gene expression;    synaptotagmin promoter;    ascidian;    neural induction;    motor neuron;    GFP;    epidermis;   

DOI  :  10.1006/dbio.2002.0584

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theascidianembryo,amodelfortheprimitivemodeofchordatedevelopment,rapidlyformsadorsalnervoussystemwhichconsistsofasmallnumberofneurons.Here,wehavecharacterizedthetranscriptionalregulationofanascidiansynaptotagmin(syt)genetoexplorethemolecularmechanismsunderlyingdevelopmentofsynaptictransmission.Insituhybridizationshowedthatsytisexpressedinallneuronsdescribedinpreviousstudiesandtransientlyintheembryonicepidermis.Neuronalexpressionofsytrequiresinductionfromthevegetalsideoftheembryo,whereasepidermalexpressionoccursautonomouslyinisolatedectodermalblastomeres.Introductionofgreenfluorescentproteinreportergeneconstructsintotheascidianembryosindicatesthatagenomicfragmentofthe3.4-kb5′upstreamregioncontainspromoterelementsofsytgene.Deletionanalysisofthepromotersuggeststhatsytexpressioninneuronsandintheembryonicepidermisdependsondistinctcis-regulatoryregions.

【 授权许可】

Unknown