SNARE Complex Assembly Is Required for Human Sperm Acrosome Reaction

Developmental Biology

Claudia N. Tomes¹  Marcela Michaut¹  Gerardo De Blas¹  Pablo Visconti¹  Luis S. Mayorga¹  Ulf Matti¹ 

[1] Laboratorio de Biologı́a Celular y Molecular, Instituto de Histologı́a y Embriologı́a (IHEM-CONICET), Facultad de Ciencias Médicas, CC 56, Universidad Nacional de Cuyo, 5500, Mendoza, Argentina

关键词: acrosome;    calcium;    exocytosis;    Rab3;    SNAP-25;    SNARE;    sperm;    syntaxin;    toxin;    VAMP;   

DOI  :  10.1006/dbio.2002.0567

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Exocytosisoftheacrosome(theacrosomereaction)isaterminalmorphologicalalterationthatspermmustundergopriortopenetrationoftheextracellularcoatoftheegg.Ca2+isanessentialmediatorofthisregulatedsecretoryevent.Aidedbyastreptolysin-Opermeabilizationprotocoldevelopedinourlaboratory,wehavepreviouslydemonstratedrequirementsforRab3A,NSF,andsynaptotagminVIinthehumanspermacrosomereaction.Interestingly,Rab3AelicitsanexocytoticresponseofcomparablemagnitudetothatofCa2+.Here,wereportadirectrolefortheSNAREcomplexintheacrosomereaction.First,thepresenceofSNAREproteinsisdemonstratedbyWesternblot.Second,theCa2+-triggeredacrosomereactionisinhibitedbybotulinumneurotoxinsBoNT/A,-E,-C,and-F.Third,antibodyinhibitionstudiesshowarequirementforSNAP-25,SNAP-23,syntaxins1A,1B,4,and6,andVAMP2.Fourth,additionofbacteriallyexpressedSNAP-25andSNAP-23abolishesexocytosis.AcrosomereactionelicitedbyRab3-GTPisalsoinhibitedbyBoNT/A,-C,and-F.Takentogether,theseresultsdemonstratearequirementformembersofallSNAREproteinfamiliesintheCa2+-andRab3A-triggeredacrosomereaction.Furthermore,theyindicatethattheonsetofspermexocytosisreliesonthefunctionalassemblyofSNAREcomplexes.

【 授权许可】

Unknown