Conserved Interactions with Cytoskeletal but Not Signaling Elements Are an Essential Aspect of Drosophila Wasp Function

Developmental Biology

Eyal D. Schejter¹  Tamar Tal¹  Dalit Vaizel-Ohayon¹ 

[1] Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, 76100, Israel

关键词: WASp;    Drosophila;    Arp2/3 complex;    CDC42;    PIP2;    sensory organs;   

DOI  :  10.1006/dbio.2002.0571

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Wiskott–AldrichSyndromeproteins(WASp)serveasimportantregulatorsofcytoskeletalorganizationandfunction.Thesemodularproteins,whicharewell-conservedamongeukaryoticspecies,acttopromoteactinfilamentassemblyinresponsetocuesfromvarioussignaltransductionpathways.GeneticanalysishasrevealedarequirementforthesingleDrosophilahomolog,Wasp(Wsp),incell-fatedecisionsgoverningspecificneuronallineages.WehaveusedthisuniquedevelopmentalcontexttoassessthecontributionsofestablishedsignalingandcytoskeletalpartnersofWASp.Wepresentbiochemicalandgeneticevidencethat,asexpected,DrosophilaWspperformsitsdevelopmentalroleviatheArp2/3complex,indicatingconservationofthecytoskeletalaspectofWspfunctioninvivo.Incontrast,wefindthatassociationwiththekeysignalingmoleculesCDC42andPIP2isnotanessentialrequirement,implyingthatactivationofWspfunctioninvivodependsonadditionaloralternativesignalingpathways.

【 授权许可】

Unknown