Tissue Origins and Interactions in the Mammalian Skull Vault

Developmental Biology

Robert E. Maxson¹  Sachiko Iseki¹  Henry M. Sucov¹  Xiaobing Jiang¹  Gillian M. Morriss-Kay¹ 

[1] Institute for Genetic Medicine, University of Southern California Keck School of Medicine, Los Angeles, California, 90033

关键词: mouse development;    neural crest;    mesoderm;    skull sutures;    Wnt1;    retinoic acid;   

DOI  :  10.1006/dbio.2001.0487

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringmammalianevolution,expansionofthecerebralhemisphereswasaccompaniedbyexpansionofthefrontalandparietalbonesoftheskullvaultanddeploymentofthecoronal(fronto-parietal)andsagittal(parietal–parietal)suturesasmajorgrowthcentres.Usingatransgenicmousewithapermanentneuralcrestcelllineagemarker,Wnt1-Cre/R26R,weshowthatbothsuturesareformedataneuralcrest–mesoderminterface:thefrontalbonesareneuralcrest-derivedandtheparietalbonesmesodermal,withatongueofneuralcrestbetweenthetwoparietalbones.BydetailedanalysisofneuralcrestmigrationpathwaysusingX-galstaining,andmesodermaltracingbyDiIlabelling,weshowthattheneuralcrest–mesodermaltissuejuxtapositionthatlaterformsthecoronalsutureisestablishedatE9.5asthecaudalboundaryofthefrontonasalmesenchyme.Asthecerebralhemispheresexpand,theyextendcaudally,passingbeneaththeneuralcrest–mesodermalinterfacewithinthedermis,carryingwiththemalayerofneuralcrestcellsthatformstheirmeningealcovering.ExposureofembryostoretinoicacidatE10.0reducesthismeningealneuralcrestandinhibitsparietalossification,suggestingthatintramembranousossificationofthismesodermalbonerequiresinteractionwithneuralcrest-derivedmeninges,whereasossificationoftheneuralcrest-derivedfrontalboneisautonomous.Theseobservationsprovidenewperspectivesonskullevolutionandonhumangeneticabnormalitiesofskullgrowthandossification.

【 授权许可】

Unknown