Developmental Biology | |
Clonal Analysis of Stomatal Development and Patterning in Arabidopsis Leaves | |
Carmen Fenoll1  Laura Serna1  Javier Torres-Contreras1  | |
[1] Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, E-45071, Toledo, Spain | |
关键词: Activator element; anisocytic stomatal complex; Arabidopsis; clockwise; clonal analysis; counterclockwise; epidermis; monoclonal; polyclonal; stomatal pattern; | |
DOI : 10.1006/dbio.2001.0506 | |
学科分类:生物科学(综合) | |
来源: Academic Press | |
【 摘 要 】
Celllineagehasbeenusedtoexplainthestomataldistributioninseveralplantspecies.Wehaveusedtransgenicplantscarryinga35SGUS::AcconstructthatproducesclonalsectorstoanalyzethepossibleroleofcelllineageduringtheestablishmentofstomatalpatterninginArabidopsisleaves.Theanalysisofsectorsrangingfromtwotoeighteencellssupportstheconclusionthatmoststomatalcomplexesderivefromasingleandimmediateprecursorcellthroughastereotypedpatternofthreeunequalcelldivisionsfollowedbyafinalequalone.Inaddition,itshowsthatthesuccessivecelldivisionstakeplaceataconstantangle(approximately60°)withrespecttothepreviousone.Interestingly,thisangulardimensionshiftsfrom60°to0°inthelastcelldivisionthatgivesrisetothestoma.Thesesectorsalsorevealthedevelopmentofbothclockwiseandcounterclockwisepatternsofcelldivisionsduringstomataldevelopmentinapproximatelyequalnumbers.Ourclonalanalysisindicatesthatcelldivisionsinvolvedinthedevelopmentofstomatalcomplexesareprobablythelastonescontributingtoepidermalgrowthanddevelopment.Finally,thestereotypedpatternofcelldivisionsthatculminatesintheformationofstomatalcomplexesindicatesthatcelllineageplaysaveryimportantroleduringstomatalpatternestablishment.
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