Clonal Analysis of Stomatal Development and Patterning in Arabidopsis Leaves

Developmental Biology

Carmen Fenoll¹  Laura Serna¹  Javier Torres-Contreras¹ 

[1] Facultad de Ciencias del Medio Ambiente, Universidad de Castilla-La Mancha, E-45071, Toledo, Spain

关键词: Activator element;    anisocytic stomatal complex;    Arabidopsis;    clockwise;    clonal analysis;    counterclockwise;    epidermis;    monoclonal;    polyclonal;    stomatal pattern;   

DOI  :  10.1006/dbio.2001.0506

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Celllineagehasbeenusedtoexplainthestomataldistributioninseveralplantspecies.Wehaveusedtransgenicplantscarryinga35SGUS::AcconstructthatproducesclonalsectorstoanalyzethepossibleroleofcelllineageduringtheestablishmentofstomatalpatterninginArabidopsisleaves.Theanalysisofsectorsrangingfromtwotoeighteencellssupportstheconclusionthatmoststomatalcomplexesderivefromasingleandimmediateprecursorcellthroughastereotypedpatternofthreeunequalcelldivisionsfollowedbyafinalequalone.Inaddition,itshowsthatthesuccessivecelldivisionstakeplaceataconstantangle(approximately60°)withrespecttothepreviousone.Interestingly,thisangulardimensionshiftsfrom60°to0°inthelastcelldivisionthatgivesrisetothestoma.Thesesectorsalsorevealthedevelopmentofbothclockwiseandcounterclockwisepatternsofcelldivisionsduringstomataldevelopmentinapproximatelyequalnumbers.Ourclonalanalysisindicatesthatcelldivisionsinvolvedinthedevelopmentofstomatalcomplexesareprobablythelastonescontributingtoepidermalgrowthanddevelopment.Finally,thestereotypedpatternofcelldivisionsthatculminatesintheformationofstomatalcomplexesindicatesthatcelllineageplaysaveryimportantroleduringstomatalpatternestablishment.

【 授权许可】

Unknown