Epithelial–Stromal Tissue Interaction in Paramesonephric (Müllerian) Epithelial Differentiation

Developmental Biology

Paul S. Cooke¹  Gerald R. Cunha¹  Takeshi Kurita¹ 

[1] Department of Anatomy, University of California, San Francisco, California, 94143-0452

关键词: uterus;    vagina;    cervix;    tissue recombination;    cornification;    estrogen receptor α;    progesterone receptor;    cytokeratins;    proliferation;   

DOI  :  10.1006/dbio.2001.0458

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Duringorganogenesis,themiddletocaudalportionofMüllerianepitheliumdifferentiatesintouterineandvaginalepitheliainfemales.Functionaldifferentiationofuterineandvaginalepitheliaoccursinadulthood,andisregulatedby17β-estradiol(E2)andprogesterone.Inthisreport,therolesofmesenchyme/stromaindifferentiationofuterineandvaginalepitheliawerestudiedintissuerecombinationexperiments.Atbirth,Müllerianepitheliumwasnegativeforuterineandvaginalepithelialmarkers.TissuerecombinantexperimentsshowedthatuterineandvaginalgeneexpressionpatternswereinducedinneonatalMüllerianepitheliumbytherespectivemesenchymes.Differentiatedadultuterineandvaginalepitheliadidnotchangetheiroriginalgeneexpressioninresponsetoheterotypicmesenchymalinduction.Intheadultvagina,E2inducedexpressionofinvolucrin,aCCAAT/enhancer-bindingproteinβandcytokeratin1viaestrogenreceptorα(ERα).Tissuerecombinationexperimentswithwild-typeandERαknockoutmicedemonstratedthatepithelialgeneexpressionisregulatedbyE2viaepithelial–stromaltissueinteractions.Uterine/vaginalheterotypictissuerecombinationsdemonstratedthatfunctionaldifferentiationofuterineandvaginalepitheliarequiredorgan-specificstromalfactors.Incontrast,stromalsignalsregulatingepithelialproliferationappearedtobenonspecificintheuterusandvagina.

【 授权许可】

Unknown