Embryonic Origin of Avian Corneal Sensory Nerves

Developmental Biology

Peter Y. Lwigale¹ 

[1]Division of Biology, Kansas State University, Ackert Hall, Manhattan, Kansas, 66506-4901

关键词: neural crest;    placode;    trigeminal ganglion;    nerves;    cornea;    quail–chick chimera;   

DOI  :  10.1006/dbio.2001.0450

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Sensorynervesplayavitalroleinmaintainingcornealtransparency.Theyoriginateinthetrigeminalganglion,whichisderivedfromtwoembryoniccellpopulations(cranialneuralcrestandectodermalplacode).Nonetheless,itisunclearwhethercornealnervesarisefromneuralcrest,fromplacode,orfromboth.Quail–chickchimerasandspecies-specificantibodiesallowedtracingquail-derivedneuralcrestorplacodecellsduringtrigeminalganglionandcornealdevelopment,andafterablationofeitherneuralcrestorplacode.Neuralcrestchimerasshowedquailnucleiintheproximalpartofthetrigeminalganglion,andquailnervesinthepericornealnerveringandinthecornea.Insharpcontrast,placodechimerasshowedquailnucleiinthedistalpartofthetrigeminalganglion,butnoquailnervesinthecorneaorinthepericornealnervering.Quailplacode-derivednerveswerepresent,however,intheeyelids.Neuralcrestablationbetweenstages8and9resultedindiminishedtrigeminalgangliaandabsenceofcornealinnervation.Ablationofplacodeafterstage11resultedinlossoftheophthalmicbranchofthetrigeminalganglionandreducedcornealinnervation.Noninnervatedcorneasstillbecametransparent.Theseresultsindicateforthefirsttimethatalthoughbothneuralcrestandplacodecontributetothetrigeminalganglion,cornealinnervationisentirelyneuralcrest-derived.Nonetheless,propercornealinnervationrequirespresenceofbothcelltypesintheembryonictrigeminalganglion.Also,completelackofinnervationhasnodiscernibleeffectondevelopmentofcornealtransparencyorcelldensities.

【 授权许可】

Unknown