FGFR3 Expression during Development and Regeneration of the Chick Inner Ear Sensory Epithelia

Developmental Biology

Olivia Bermingham-McDonogh¹  Thomas A. Reh¹  Jennifer S. Stone¹  Edwin W Rubel¹ 

[1] Virginia Merrill Bloedel Hearing Research Center, University of Washington School of Medicine, Box 357420, Seattle, Washington, 98195

关键词: Cek2;    basilar papilla;    chicken;    hair cell;    growth factors;    receptors;    cochlea;   

DOI  :  10.1006/dbio.2001.0412

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Severalstudiessuggestfibroblastgrowthfactorreceptor3(FGFR3)playsaroleinthedevelopmentoftheauditoryepitheliuminmammals.WeundertookastudyofFGFR3inthedevelopingandmaturechickeninnerearandduringregenerationofthisepitheliumtodeterminewhetherFGFR3showsasimilarpatternofexpressioninbirds.FGFR3mRNAishighlyexpressedinmostsupportcellsinthematurechickbasilarpapillabutnotinvestibularorgansofthechick.Thegeneisexpressedearlyinthedevelopmentofthebasilarpapilla.Gentamicintreatmentsufficienttodestroyhaircellsinthebasilarpapillacausesarapid,transientdownregulationofFGFR3mRNAintheregionofdamage.Intheinitialstagesofhaircellregeneration,thesupportcellsthatreenterthemitoticcycleinthebasilarpapilladonotexpressdetectablelevelsofFGFR3mRNA.However,oncethehaircellshaveregeneratedinthisregion,thelevelsofFGFR3mRNAandproteinexpressionrapidlyreturntoapproximatethoseintheundamagedepithelium.TheseresultsindicatethatFGFR3expressionchangesafterdrug-inducedhaircelldamagetothebasilarpapillainanoppositewaytothatfoundinthemammaliancochleaandmaybeinvolvedinregulatingtheproliferationofsupportcells.

【 授权许可】

Unknown