Dental Pulp Cells Produce Neurotrophic Factors, Interact with Trigeminal Neurons in Vitro, and Rescue Motoneurons after Spinal Cord Injury

Developmental Biology

Christopher A. Nosrat¹  Irina V. Nosrat¹  Johan Widenfalk¹  Lars Olson¹ 

[1] Laboratory of Oral Neurobiology, Department of Biologic and Materials Sciences, University of Michigan School of Dentistry, Ann Arbor, Michigan, 48109

关键词: neurotrophin;    development;    tooth innervation;    motoneuron survival;    NGF;    BDNF;    GDNF;    transplantation;    intraocular;   

DOI  :  10.1006/dbio.2001.0400

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Interactionsbetweeningrowingnervefibersandtheirtargettissuesformthebasisforfunctionalconnectivitywiththecentralnervoussystem.Studiesofthedevelopingdentalpulpinnervationbynervefibersfromthetrigeminalganglionisanexcellentexampleofnerve–targettissueinteractionsandwillallowspecificquestionsregardingdevelopmentofthedentalpulpnervesystemtobeaddressed.Dentalpulpcells(DPC)produceanarrayofneurotrophicfactorsduringdevelopment,suggestingthattheseproteinsmightbeinvolvedinsupportingtrigeminalnervefibersthatinnervatethedentalpulp.Wehaveestablishedaninvitroculturesystemtostudytheinteractionsbetweenthedentalpulpcellsandtrigeminalneurons.Weshowthatdentalpulpcellsproduceseveralneurotrophicfactorsinculture.WhenDPCarecoculturedwithtrigeminalneurons,theypromotesurvivalandaspecificandelaborateneuriteoutgrowthpatternfromtrigeminalneurons,whereasskinfibroblastsdonotprovideasimilarsupport.Inaddition,weshowthatdentalpulptissuebecomesinnervatedwhentransplantedectopicallyintotheanteriorchamberoftheeyeinrats,andupregulatesthecatecholaminergicnervefiberdensityoftheirises.Interestingly,graftingthedentalpulptissueintohemisectedspinalcordincreasesthenumberofsurvivingmotoneurons,indicatingafunctionalbioactivityofthedentalpulp-derivedneurotrophicfactorsinvivobyrescuingmotoneurons.Basedonthesefindings,weproposethatdentalpulp-derivedneurotrophicfactorsplayanimportantroleinorchestratingthedentalpulpinnervation.

【 授权许可】

Unknown