The Drosophila JNK Pathway Controls the Morphogenesis of the Egg Dorsal Appendages and Micropyle

Developmental Biology

Norbert Perrimon¹  Magali Suzanne¹  Stéphane Noselli¹ 

[1] Centro de Biologı́a Molecular “Severo Ochoa” Facultad de Ciencias, Universidad Autónoma de Madrid, 28049, Madrid, Spain

关键词: JNK;    hep;    morphogenesis;    epithelium;    oogenesis;    follicle cells;    appendages;    micropyle;   

DOI  :  10.1006/dbio.2001.0384

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

DuringDrosophilaoogenesis,theformationoftheeggrespiratoryappendagesandthemicropylerequiretheshapingofanterioranddorsalfolliclecells.Priortotheirmorphogenesis,cellsofthepresumptiveappendagesaredeterminedbyintegratingdorsal–ventralandanterior–posteriorpositionalinformationprovidedbytheepidermalgrowthfactorreceptor(EGFR)andDecapentaplegic(Dpp)pathways,respectively.Weshowherethatanothersignalingpathway,theDrosophilaJun-N-terminalkinase(JNK)cascade,isessentialforthecorrectmorphogenesisofthedorsalappendagesandthemicropyleduringoogenesis.MutantfolliclecellclonesofmembersoftheJNKpathway,includingDJNKK/hemipterous(hep),DJNK/basket(bsk),andDjun,blockdorsalappendageformationandaffectthemicropyleshapeandsize,suggestingalaterequirementfortheJNKpathwayinanteriorchorionmorphogenesis.Insupportofthisview,hepdoesnotaffectearlyfolliclecellpatterningasindicatedbythenormalexpressionofkekkon(kek)andBroad-Complex(BR-C),twoofthetargetsoftheEGFRpathwayindorsalfolliclecells.Furthermore,theexpressionoftheTGF-βhomologdpp,whichisunderthecontrolofhepinembryos,isnotcoupledtoJNKactivityduringoogenesis.Weshowthathepcontrolstheexpressionofpuckered(puc)inthefollicularepitheliuminacell-autonomousmanner.SincepucoverexpressionintheeggfollicularepitheliummimicsJNKappendagesandmicropylephenotypes,itindicatesanegativeroleofpucintheirmorphogenesis.TheroleoftheJNKpathwayinthemorphogenesisoffolliclecellsandotherepitheliaduringdevelopmentisdiscussed.

【 授权许可】

Unknown