IGFs, Insulin, Shh, bFGF, and TGF-β1 Interact Synergistically to Promote Somite Myogenesis in Vitro

Developmental Biology

Stephen D. Hauschka¹  Julie C. Kiefer¹  Asta Pirskanen¹ 

[1] Department of Biochemistry, University of Washington, Box 357350, Seattle, Washington, 98195

关键词: somite;    myogenesis;    muscle;    neural tube;    insulin-like growth factors (IGFs);    IGF receptors;    Sonic hedgehog (Shh);    fibroblast growth factor;    TGF-β1;    apoptosis;   

DOI  :  10.1006/dbio.2000.9784

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

StudiesfromourgroupandothershaveshownthatinvitrosomitemyogenesisisregulatedbyneuraltubeandnotochordfactorsincludingWnt,Sonichedgehog(Shh),andbasicfibroblastgrowthfactor(bFGF)togetherwithtransforminggrowthfactor-β1(TGF-β1).Inthisstudywereportthatinsulinandinsulin-likegrowthfactorsIandII(IGF-Iand-II)alsopromotemyogenesisinexplantculturescontainingsinglesomitesorsomite-sizedpiecesofsegmentalplatemesodermfrom2-day(stage10–14)chickenembryosandthatthecombinationofinsulin/IGFswithbFGFplusTGF-β1promotesevenhigherlevelsofmyogenesis.WealsofoundthatShhpromotesmyogenesisinthisinvitrosystemandthatShhinteractssynergisticallywithinsulin/IGFstopromotehighlevelsofmyogenesis.RT-PCRanalysisdetectedinsulin,IGF-II,insulinreceptor,andIGFreceptormRNAsinboththeneuraltubeandthesomites,whereasIGF-Itranscriptsweredetectedinentireembryosbutnotintheneuraltubeorsomites.Treatmentofsomite–neuraltubecocultureswithanti-insulin,anti-IGF-II,anti-insulinreceptor,oranti-IGFreceptorblockingantibodiescausedasignificantdecreaseinmyogenesis.TheseresultsareconsistentwiththehypothesisthatsystemicIGF-IaswellasinsulinandIGF-IIsecretedbytheneuraltubeactasadditionalearlymyogenicsignalsduringembryogenesis.Furtherstudiesindicatethatinsulin,IGFs,bFGF,andShhalsostimulatesomitecellproliferationandinfluenceapoptosis.

【 授权许可】

Unknown