Transforming growth factor β1 is an epithelial-derived signal peptide that influences otic capsule formation☆

Developmental Biology

Dorothy A. Frenz¹  Kathleen C. Flanders¹  Vera Galinovic-Schwartz¹  Wei Liu¹  Thomas R. Van de Water¹ 

[1] Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, New York 10461 USA

DOI  :  10.1016/0012-1606(92)90117-Y

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Interactionsbetweenepithelialandmesenchymaltissuesinthedevelopinginnereardirecttheformationofitscartilaginouscapsule.Recentworkindicatesthatmanygrowthfactorsaredistributedintheearlyembryoinvivoinatemporal-spatialpatternthatcorrelateswithsitesofongoingmorphogeneticevents.Wereportherethelocalizationoftransforminggrowthfactorβ1(TGF-β1inbothepithelialandmesenchymaltissuesofthemouseinnerearbetween10and16daysofembryonicdevelopment(E10–E16).Inaddition,utilizingahigh-densityculturesystemasaninvitromodelofoticcapsulechondrogenesis,weshowthatmodulationofchondrogenesisbyTGF-β1inculturedmouseperioticmesenchymemimicstheinvitroeffectsofoticepitheliumontheexpressionofchondrogenicpotential.WeprovideevidenceofacausalrelationshipofthisgrowthfactortooticcapsuleformationinsitubydemonstratingthattheactualsequenceofchondrogeniceventsthatoccurinthedevelopingembryoisreproducedinculturebytheadditionofexogenousTGF-β1peptide.Furthermore,inculturesofmesenchymecontainingoticepithelium,wedemonstratethelocalizationofendogenousTGF-β1,firstwithintheepithelialtissueandlaterwithinboththeepitheliumanditssurroundingperioticmesenchyme,contrastedtoanabsenceofendogenousTGF-β1inculturesofmesenchymealone.OurresultssuggestthatTGF-β1isoneofthesignalmoleculesthatmediatetheeffectsofoticepitheliumininfluencingtheformationofthecartilaginousoticcapsule

【 授权许可】

Unknown