Evidence for participation of sperm proteinases in fertilization of the solitary ascidian, Halocynthia roretzi: Effects of protease inhibitors☆

Developmental Biology

Motonori Hoshi¹  Takaharu Numakunai¹  Hitoshi Sawada¹ 

[1]Biochemical Laboratory, Institute of Low Temperature Science, Hokkaido University, Sapporo 060, Japan

DOI  :  10.1016/0012-1606(81)90322-5

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Effectsof15proteinaseinhibitorsandaninhibitoragainstaminopeptidasesonfertilizationofthesolitaryascidian,Halocynthiaroretziwerestudiedinsearchoflysins.Fertilizationofintacteggswasblockedbythreetrypsininhibitors,leupeptin,antipain,andsoybeantrypsininhibitor,andbytwochymotrypsininhibitors,chymostatinandpotatoproteinaseinhibitorI.Ontheotherhand,thefertilizationofnakedeggswasnotblockedatallbyleupeptinandwasonlypartiallyblockedbychymostatinattheconcentrationssufficientforblockingthatofintacteggs.Thisindicatesthatspermatozoautilizetrypsin-likeandchymotrypsin-likeproteinasesprobablyaslysinsforpenetratingthroughthechorion.Thechymotrypsin-likeactivityappearstobealsorequiredforsomestepbesidesspermpenetrationthroughtheegginvestments.

【 授权许可】

Unknown