Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa☆

Developmental Biology

Richard O. Hynes¹  Byrne W. Mayer Jr.¹  Elizabeth D. Hay¹ 

[1] Department of Anatomy, Harvard Medical School, Boston, Massachusetts 02115 USA

关键词: anti-R;    goat anti-rabbit IgG;    CSP;    cell surface protein;    DAB;    diaminobenzidine;    ECM;    extracellular matrix;    Fab;    the antibody-binding fragment of IgG;    Fc;    the non-antibody binding fragment of IgG;    Fe-anti-R;    ferritin-conjugated goat anti-rabbit IgG;    GAG;    glycosaminoglycans;    IgG;    immunoglobulin G;    LETS;    large;    external;    transformation-sensitive protein;    PAP;    peroxidase anti-peroxidase;    PBS;    phosphate-buffered saline (pH 7.2);    Ranti-FN;    rabbit antifibronectin antiserum;    RR;    ruthenium red;    SDS;    sodium dodecyl sulfate;    SFA;    fibroblast surface antigen;    TBS;    Tris-buffered saline;   

DOI  :  10.1016/0012-1606(81)90451-6

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Fibronectinhasbeenshowntoenhancecellmigrationinvitro.Itseemsofconsiderableinterest,therefore,tomapitsdistributionintheactualembryoduringperiodsofcellmigration.Inthisinsitustudy,weusedimmunohistochemistryattheelectronmicroscopicleveltolocalizefibronectininthreeregionsofthechickembryothatarecharacterizedbyactualorimpendingmorphogeneticmovements:dorsaltrunk(neuralcrest),ventraltrunk,andareavasculosa.Theextracellularmatrixofthe2-day-oldembryocontainsnonstriatedfibrils(5–10nmindiameter)andso-calledinterstitialbodies,bothofwhichlabelbyantibodiestofibronectinwiththeimmunoperoxidase(PAP)techniqueandwiththeimmunoferritintechnique.Inaddition,cellsurfacesandbasallaminaearestrainedbyPAPfollowingtreatmentwithantifibronectin.Usingrutheniumredtopreserveproteoglycan(PG),wewereabletodemonstrateacloseassociationofPGgranuleswiththeperipheryofthefibronectin-richinterstitialbodies.Theseresultsarethefirsttoindicatethepossiblechemicalcompositionofinterstitialbodiesandtheyraisethepossibilitythattheseuniquecomponentsofembryonicmatricesareimportantsubstratesforcellmigration.Indeed,cellprocessesofneuralcrestandothermesenchymemakeclosecontactswithinterstitialbodies.Intheareavasculosa,interstitialbodiesareconcentratedattheleadingedgeofthevascularmesenchyme.Thereis,however,noobviousgradientofextracellularfibronectininneuralcrestpathwaysorintheventraltrunk.

【 授权许可】

Unknown