Two erythropoietic microenvironments and two larval red cell lines in bullfrog tadpoles☆

Developmental Biology

Guy M. Johnson¹  Robert H. Broyles¹  Phillip B. Maples¹  Gary R. Kindell¹ 

[1] Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190 USA

关键词: EGTA;    ethyleneglycol bis(β-aminoethyl ether) N;    N′-tetraacetic acid;    Hb;    hemoglobin;    Hepes;    N-2-hydroxyethyl piperazine-N′-2-ethanesulfonic acid;    RBC;    red blood cell;   

DOI  :  10.1016/0012-1606(81)90293-1

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

PreviousworkhasshownthatRanacatesbeianatadpoleshavetwoerythropoieticsites:mesonephrickidneyandliver.Bothsitesapparentlyareactivethroughoutlarvallife.Amongthepleomorphiclarvalredbloodcells(RBCs)weobservetwomorphologicaltypeswhichdifferinthelarvalhemoglobins(Hb)thattheycontain.Onetypeisanoblong,ovalRBCwithanacentricnucleusandcontainsthelarvalHbwhichisslowestmigratingonelectrophoresisatbasicpH.ThisRBCtypeemanatesfromthekidneys.Theothertype,whichisellipticalinshape,withacentralnucleus,containsthreeotherlarvalHbsandemanatesfromtheliver.Histologicalsectionsofkidneyandliverrevealerythropoieticfociofdifferingcellularcompositionandconfiguration.WehavehypothesizedthatchoiceofRBCandHbtypesisdeterminedinpartbythedifferenterythropoieticmicroenvironments.Insupportofthishypothesisisourfindingthat,inorgancultures,livercoculturedwithkidneyundergoesashiftinitsHbpattern.PartoftheshiftistowardtheHbtypewhichistheproductofkidneyerythropoiesis.

【 授权许可】

Unknown