Studies on amphibian yolk: VIII. The estrogen-induced hepatic synthesis of a serum lipophosphoprotein and its selective uptake by the ovary and transformation into yolk platelet proteins in Xenopus laevis☆☆☆

Developmental Biology

Robin A. Wallace¹  Donald W. Jared¹ 

[1] Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830 USA

关键词: HCG;    human chorionic gonadotropin;    SLPP;    serum lipophosphoprotein;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0012-1606(69)90085-2

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

14C-labeledleucineinjectedintoXenopusrapidlybecomesincorporatedintoliverprotein;subsequentlythespecificactivityofserumproteinrises.Theextentofserumproteinlabelingispronounced,particularlyinestrogen-treatedmalesandvitellogenicfemales;however,inthelattercase,labeledproteinrapidlydisappearsfromthecirculationandthespecificactivityofovarianproteinsimultaneouslyrisestoaplateau.Serumchromatogramsdemonstratethatmostofthevariablelabelingoftheserumisthefunctionofasingleserumcomponent,designatedSLPP.Thiscomponentisproducedbytheliverasindicatedbythesyntheticcapacityofliverslicesinvitro,andthehepaticsynthesisandsecretionofSLPPappearstobeunderthecontrolofestrogen.SLPPistakenupselectivelybythematuringovaryandspecificallybythevitellogenicoocyte.Thelatter,however,mustfirstbeactivatedbyahormonalfactorinordertoperformthistask.Onceincorporatedintotheoocyte,SLPPappearstotransformintothetwoproteinsofthecrystallineyolkplatelet,phosvitinandlipovitellin,andhencerepresentstheprecursormoleculeforcrystallineyolk.

【 授权许可】

Unknown