Inhibition of cell division and cell elongation in higher plants by inhibitors of DNA synthesis☆

Developmental Biology

Anton Lang¹  Joseph Nitsan¹ 

[1] Division of Biology, California Institute of Technology, Pasadena, California USA

关键词: GA3;    gibberellin A3;    FUDR;    5-fluorodeoxyuridine;    MC;    mitomycin C;    PEA;    phenethyl alcohol;   

DOI  :  10.1016/0012-1606(65)90003-5

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Lettuceseedlingswereincubatedinthepresenceandabsenceofgibberellin(GA3)insolutionscontainingdifferentinhibitorsofDNAsynthesis.GA3promotedtheelongationofthelettucehypocotylinthedark,andofbothhypocotylandcotyledonsinthelight;50%growthinhibitionwasobtainedby3×10−7M5-fluorodeoxyuridine(FUDR).InhibitionproducedbyFUDRat10−7to10−5Mwascompletelyreversedby10−4Mthymidine,butnotbyuridine.Theinhibitionproducedbyamethopterinwasonlypartlyreversedbythymidine;50%inhibitionwasobtainedat10−7Mofamethopterin,10−5MofmitomycinC,and2×10−3Mofphenethylalcohol.Intheinhibitedtissuesbothcelldivisionandcellelongationweresuppressed.Inepicotylsofdecapitatedlentilseedlings,whichgrowmainlybycellelongation,cellelongationwaspromotedbyGA3andwasinhibitedbyFUDRat10−7to10−5M.Thisinhibitionwasagainreversedbythymidine,butnotbyuridine.ThepossiblerelationshipbetweenDNAsynthesisandcellelongationinGA3-promotedtissuesisdiscussed.

【 授权许可】

Unknown