High Time Resolution Time-Lapse Imaging Reveals Continuous Existence and Rotation of Stress Fibers under Cyclic Stretch in HUVEC

Journal of Biomechanical Science and Engineering

Yusuke MITSUOKA³  Katsuko S. FURUKAWA¹  Akira TSUKAMOTO²  Takashi USHIDA³  Shunsuke IWAYOSHI³ 

[1] Department of Bioengineering, Graduate School of Engineering, The University of Tokyo;Depatment of Applied Physics, National Defense Academy;Department of Mechanical Engineering, Graduate School of Engineering, The University of Tokyo

关键词: Cyclic Stretch;    Stress Fiber;    Orientation Dynamics;    Morphological Change;    Time-Lapse Imaging;    HUVEC;   

DOI  :  10.1299/jbse.7.188

来源: Japan Society of Mechanical Engineers

【 摘 要 】

References(29)Cited-By(1)Cells under cyclic stretch sense their environments and induce responses such as actin stress fiber (SF) reorientation and morphological changes. These physiological responses are thought to occur when cells sense incompatibility between SF orientation and stretching direction. This hypothesis requires existence of SFs. However, such existence of SFs in cells under cyclic stretch remains unclear since few studies attempted to track the existence of SFs throughout cyclic stretch. In order to track the existence of SFs throughout cyclic stretch, high time resolution time-lapse imaging was improved in two points. First, SFs were clearly imaged with coexpression of DsRed-zyxin and GFP-actin. Second, time resolution was improved so that fluorescence images were obtained every 28 sec. With the improved high time resolution time-lapse imaging, it was revealed, for the first time, that SFs could exist continuously throughout cyclic stretch. Moreover, physiological responses including morphological change as well as SF reorientation occurred during the time when SFs formed incompatibility between SF orientation and stretching direction. These results demonstrated that SFs continuously existed in cells under cyclic stretch and in turn suggested that continuous presence of incompatibility between orientation of long-lasting SFs and the stretching direction might be important for mechanosensing which induces physiological responses.

【 授权许可】

Unknown