Proliferation of Periodontal Ligament Cells on Biodegradable Honeycomb Film Scaffold with Unified Micropore Organization

Journal of Biomechanical Science and Engineering

Hiroshi ISHIHATA²  Masaru NAGAMINE⁴  Mitsuru SHIMONISHI¹  Nagayoshi IWAMA²  Hidetoshi SHIMAUCHI²  Masaru TANAKA³  Eiji NEMOTO²  Noriaki MURAKAMI²  Masatsugu SHIMOMURA³  Sousuke KANAYA²  Masahiro ARA³ 

[1] Graduate School of Dentistry, Division of Comprehensive Dentistry, Tohoku University;Graduate School of Dentistry, Division of Periodontology and Endodontology, Tohoku University;Institute of Multidisciplinary Research for Advanced Materials, Tohoku University;Nagamine Manufacturing Co., Ltd.

关键词: Self-organization;    Biomaterials;    Scaffold;    Periodontal Ligament;    Cell Adhesion;    Tissue Regeneration;   

DOI  :  10.1299/jbse.5.252

来源: Japan Society of Mechanical Engineers

【 摘 要 】

References(22)Cited-By(3)Tissue-engineered grafts using a scaffold can be used in treating periodontal disease; however, previous scaffolds for the cultivations of the periodontal ligament cells have been structurally incompatible with the morphological requirements of human periodontal tissue. Here, we describe a self-organized honeycomb-patterned film (honeycomb film) that acted as an appropriate scaffold for periodontal tissue regeneration. The honeycomb films were prepared from biodegradable poly(ε-caprolactone) with highly regular three-dimensional micropatterned surface topography by casting a polymer solution of water-immiscible solvent under humid conditions. To evaluate its performance in activating the proliferation and organizing of cells, we have demonstrated specific behaviors of the cultured periodontal ligament cells on the self-organized honeycomb structures in vitro. Fibroblast-like cells derived from the periodontal ligament of extracted human molar teeth were cultivated on three types of honeycomb films with 5-, 10-, and 15-µm pore sizes for 4 h to 42 d. Morphological observation of the cultured tissues at 4—72 h revealed that the pseudopodiums of cell bodies were attached to the pillars in the honeycomb structure. A certain number of cells shifted their cell bodies into the honeycomb structural lumen through the oscula of 10- and 15-µm pores. After 28 and 42 d, the cells were observed to have formed multiple layers; further, each cell had penetrated through the 10- and 15-µm pores in the honeycomb film. The morphological examination of the honeycomb film along with the pillar structures revealed that the scaffold was clusteringly arrayed with interconnected structures, remarkably enhanced proliferation, and extension of the cultured cells. We consider that the film can be applied in periodontal therapy for use as a scaffold for periodontal tissue regeneration.

【 授权许可】

Unknown