Interaction between Human Serum Albumin and Different Anatase TiO2 Nanoparticles: A Nano-bio Interface Study

Nanomaterials and Nanotechnology

Mariafrancesca Cascione¹  Barbara Federica Scremin¹  Claudia Carlucci¹  Cinzia Citti¹  Viviana Vergaro¹  Francesca Conciauro¹  Giuseppe Cannazza¹  Paolo Maria Congedo¹  Caterina Lorusso¹  Giuseppe Ciccarella¹ 

关键词: TiO2;    Nanocrystals;    Albumin;    Interface;    Adhesion;    ζ-potential;   

DOI  :  10.5772/61092

来源: InTech

【 摘 要 】

In this investigation, differently shaped and surface functionalized TiO2 anatase nanoparticles and human serum albumin (HSA) were selected to study protein-nanoparticles interaction both in a solution and on flat surfaces, thereby mimicking a medical device. Anatase nanocrystals were characterized by transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) surface analysis and dynamic light scattering (DLS). The protein-nanoparticles' interactions and their eventual reversibility were studied by pH dependent ζ- potential measurements in different media: ultra-pure water, a phosphate buffer simulating physiological conditions and in a culture medium supplemented with foetal bovine serum. The protein corona masking effect was evidenced and the interaction HSA-nanocrystals resulted irreversible. The interaction HSA-silicon supported TiO2 nanocrystals films was studied by atomic force microscopy (AFM), and resulted driven by the substrate hydrophilicity degree plus was different for the diverse r...

【 授权许可】

Unknown