期刊论文详细信息
Genes and Environment
Mutagenicity and NO Formation from UVA Irradiated N-Nitrosoproline, and Characterization of Photoproduct Formed in UVA-irradiated Mixture of N-Nitrosoproline with 2′-Deoxyguanosine in Neutral Solution
Kayoko Sano1  Toshinori Suzuki3  Noriko Tanaka1  Sachiko Kimura2  Shuhei Aoyama1  Chiharu Asahi1  Sakae Arimoto-Kobayashi1  Keinosuke Okamoto1  Tomoe Negishi1  Masaki Machida1 
[1] Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University;School of Human Science and Environment, University of Hyogo;Faculty of Pharmaceutical Sciences, Shujitsu University
关键词: photoreaction;    N-nitrosamine;    DNA adduct;    8-oxo-deoxyguanosine;    NO radical;   
DOI  :  10.3123/jemsge.2014.004
学科分类:分子生物学,细胞生物学和基因
来源: Japanese Environmental Mutagen Society / Nihon Kankyo Hen igen Gakkai
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【 摘 要 】

References(29)Cited-By(1)Earlier studies in our laboratory showed that UVA-irradiated N-nitrosoproline (NPRO) could produce mutagenic compounds and strand breaks in treated DNA with the release of nitric oxide and active oxygen species. In the present study, we investigated the phosphate-dependence of photomutagenicity and solvent effects on nitric oxide release from irradiated NPRO. We also investigated the UVA-driven reaction of NPRO with 2′-deoxyguanosine (dG) in neutral solution, and identified the chemical structure of the photoproduct. A mixture of NPRO and dG dissolved in neutral solution was irradiated with UVA at 320-400 nm for 6 h, and the solution was then subjected to HPLC analysis. The photoproduct isolated from the NPRO and dG mixture in neutral buffer was identified as 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG). We propose a photoreaction mechanism involving the absorption of UVA photons by NPRO followed by UVA-driven photolysis of NPRO, and subsequent production of active oxygen radicals that generate 8-oxodG.

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