New Method of Specimen Preparation as Cell Suspension for a Micronucleus Test

Genes and Environment

Hironao Takasawa²  Shigenori Minowa²  Shuichi Hamada²  Munehiro Nakagawa²  Hitoshi Saitou²  Masayoshi Kawabata²  Madoka Nakajima¹ 

[1] Biosafety Research Center, Foods, Drugs and Pesticides;Kashima Laboratory, Mitsubishi Chemical Safety Institute Ltd.

关键词: micronucleus test;    acridine orange;    smeared method;    cell suspension method;    bone marrow;    peripheral blood;   

DOI  :  10.3123/jemsge.29.100

学科分类：分子生物学，细胞生物学和基因

来源: Japanese Environmental Mutagen Society / Nihon Kankyo Hen igen Gakkai

【 摘 要 】

References(11)Cited-By(7)A new specimen preparation method named “cell suspension method” for in vivo micronucleus test is proposed. Generally, a specimen of the micronucleus test is prepared by smearing the sample cells on a slide glass before staining with Giemsa or acridine orange (A.O.). In this new method, the sample cells are suspended with formalin, and stained with A.O., then put on a slide glass and examined under a microscope without smearing. This method is more convenient than the conventional method in consideration of preparation and observation of specimens. Furthermore, preparation is repeatable after the observation in this method, the specimen is suitable for a long storage, and this method is applicable to specimen preparation using peripheral blood.

【 授权许可】

Unknown