Daily Expression of mRNAs for the Mammalian Clock Genes Per2 and Clock in Mouse Suprachiasmatic Nuclei and Liver and Human Peripheral Blood Mononuclear Cells

The Japanese Journal of Pharmacology

Hiroshi Takane²  Hisashi Suyama³  Masanori Miyata³  Eiji Shimizu³  Takanori Sako³  Hideyuki Terazono¹  Naoto Burioka³  Shigehiro Ohdo¹  Yasushi Fukuoka³  Katsuyuki Tomita³  Miyako Takata³ 

[1] Department of Clinical Pharmacokinetics, Graduate School of Pharmaceutical Sciences, Kyushu University;Department of Pharmacology, Faculty of Medicine, Tottori University;Third Department of Internal Medicine, Faculty of Medicine, Tottori University

关键词: Clock gene;    Liver;    Per2;    Peripheral blood mononuclear cell;    Suprachiasmatic nucleus;   

DOI  :  10.1254/jjp.90.263

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(30)Cited-By(35)The mammalian circadian clock is located in the suprachiasmatic nucleus (SCN) of the hypothalamus and in most peripheral tissues. Clock genes drive the biological clock. However, circadian expression variations of the human clock genes are still unclear. In this study, we analyzed the daily variations of mPer2 and mClock mRNA expression in both the mouse SCN and liver to evaluate the central and peripheral alterations in the rodent clock genes. We also examined whether there are the daily variations of the clock genes hPer2 and hClock in human peripheral blood mononuclear cells (PBMCs). The daily variation of mClock and mPer2 mRNA expression in mouse SCN and liver were determined at ZT2, ZT6, ZT10, ZT14, ZT18 or ZT22. We isolated PBMCs from 9 healthy volunteers at 9:00 and 21:00 and examined the expression of hPer2 and hClock mRNA by RT-PCR analysis. The animals exhibited a robust daily rhythm in the RNA levels of mPer2 in the SCN and liver (P<0.01, respectively). In humans, hPer2 mRNA expression also had daily variation, and the hPer2 mRNA levels at 9:00 were significantly larger than those at 21:00 (P<0.01). While, the Clock mRNA in both mice and humans exhibited no daily variation. These findings suggest that the variation in hPer2 mRNA expression may be useful for assessing human peripheral circadian systems.

【 授权许可】

Unknown