An Involvement of Granulocyte Medullasin in Phenytoin-Induced Gingival Overgrowth in Rats

The Japanese Journal of Pharmacology

Yukio Ozaki⁴  Ihachi Kato⁴  Kazushi Kunimatsu⁴  Kenji Yamamoto³  Yuzo Kato²  Yoshitaka Hara⁴  Yosuke Aoki¹ 

[1] Department of Food and Health Sciences, Jissen Women’s University Faculty of Life Sciences;Department of Pharmacology, Nagasaki University School of Dentistry;Department of Pharmacology, Kyushu University Graduate School of Dental Sciences;Department of Periodontology, Nagasaki University School of Dentistry

关键词: Medullasin;    Serine proteinase;    Phenytoin;    Gingival overgrowth;    Immunohistochemistry;   

DOI  :  10.1254/jjp.89.235

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(27)Cited-By(2)To investigate the relationship between histological changes and distributions of medullasin, a neutrophil elastase-like serine proteinase, in phenytoin-induced gingival overgrowth, we established a rat model of gingival overgrowth. Thirty-two, 20-day-old male Fischer 344 rats were fed a diet containing phenytoin and sacrificed at 1, 2, 4 and 8 weeks. Control rats (n = 40) were fed the same diet, but without the drug and killed at the same weeks as experimental rats (n = 32) and 0 week (n = 8). The mandible specimens were resected and sectioned bucco-lingually between the first and second molars. A marked inflammatory-cell infiltration and elongated rete pegs were seen in the phenytoin-treated group. The extent of the overgrowth assessed by computer image analysis and the density of medullasin-positive cells by immunohistochemistry in the approximal gingiva showed a significant increase in the phenytoin-treated group compared to the control group. A marked infiltration of the positive cells in experimental rats was observed as early as 2 weeks when gingival overgrowth was not fully established. Medullasin-positive cells were mostly neutrophils and partly macrophage-like cells. These findings suggest that medullasin may be involved in mainly host defense and secondarily collagen metabolism in the phenytoin-induced rat model of gingival overgrowth.

【 授权许可】

Unknown