Acidosis Inhibits Gallbladder Contraction Mediated by Protein Kinase C Activation

The Japanese Journal of Pharmacology

Nobuo Iwata²  Ichiro Wakabayashi¹  Hidehisa Masui³  Yasuro Kataoka¹ 

[1] Department of Hygiene and Preventive Medicine, School of Medicine, Yamagata University;2nd Department of Internal Medicine, Hyogo College of Medicine;Department of Public Health, Hyogo College of Medicine

关键词: Gallbladder contraction;    Extracellular pH;    Protein kinase C;   

DOI  :  10.1254/jjp.87.158

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(1)The effects of extracellular acidosis on gallbladder contraction were investigated using gallbladder strips isolated from guinea pigs. In an acidic medium (pH 6.9), gallbladder contraction induced by histamine and prostaglandin E2 was significantly lower than that in a normal medium (pH 7.4). Acidosis affected neither gallbladder contraction induced by histamine in the absence of extracellular Ca2+ nor that induced by KCl. Acidosis significantly inhibited Ca2+-induced contraction in the presence of sodium fluoride and phorbol 12,13-dibutyrate but not that in the presence of KCl. Staurosporine (30 nM) significantly inhibited gallbladder contraction induced by histamine and prostaglandin E2, but not that by KCl. Histamine-induced contraction in the presence of staurosporine was not affected by acidosis. Acidosis significantly inhibited Ca2+-induced contraction in the presence of histamine but not that in the presence of both histamine and staurosporine. These results suggest that extracellular acidosis selectively inhibits gallbladder contraction mediated by protein kinase C activation.

【 授权许可】

Unknown