D-Galactose-Specific Sea Urchin Lectin Sugar-Specifically Inhibited Histamine Release Induced by Datura stramonium Agglutinin: Differences Between Sugar-Specific Effects of Sea Urchin Lectin and Those of D-Galactose- or L-Fucose-Specific Plant Lectins

The Japanese Journal of Pharmacology

Hideyuki Nakagawa¹  Tamiko Suzuki-Nishimura²  Masaatsu K. Uchida² 

[1] Department of Life Sciences, University of Tokushima;Department of Pharmacology, Meiji Pharmaceutical University

关键词: Mast cell;    Histamine release;    Datura stramonium agglutinin;    D(+)Galactose-specific lectin;    Compound 48/80;    Substance P;   

DOI  :  10.1254/jjp.85.443

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(29)Cited-By(5)A new sea urchin lectin from Toxopneustes pileolus, is D(+)galactose (Gal)-, D(+)fucose (Fuc)-specific. Incubation of rat peritoneal mast cells with the lectin in the presence of 0.3 mM CaCl2 for 10 min significantly and dose-dependently inhibited the histamine release induced by N-acetyl glucosamine (GlcNAc)-specific Datura stramonium agglutinin (DSA), an activator of the Gi-protein-dependent pathway in mast cells. This inhibition by the sea urchin lectin was sugar-specifically reversed in the presence of D(+)Gal or D(+)Fuc but not L(−)Fuc. The sea urchin lectin had no effect on the histamine release induced by compound 48/80, slightly inhibited the histamine release induced by substance P and mastoparan, and slightly enhanced the histamine release induced by melittin, but these effects were not dose-dependent. Compound 48/80, substance P, mastoparan and melittin are mast cell activators without sugar residues. It is suggested that the lectin binds to D(+)Gal residues of DSA to interfere with mast cell activation induced by DSA, a glycoprotein with arabinose and Gal residues. The effects of plant lectins with affinity to D(+)Gal, N-acetyl galactosamine and/or sialic acid and L(−)Fuc on the histamine release induced by DSA, compound 48/80 and substance P were also examined.

【 授权许可】

Unknown