Non-competitive Inhibition of Kainate-Induced Currents by Diethylstilbestrol in Acutely Isolated Mouse CA1 Hippocampal Neurons

The Japanese Journal of Pharmacology

Kazuo Takahama¹  Hitoshi Ishibashi¹  Hideaki Shimada¹  Satoshi Okuya¹ 

[1] Department of Hygienic Chemistry,Faculty of Pharmaceutical Sciences,Kumamoto University,5-1 Oe-Honmachi,Kumamoto 862-0973,Japan

关键词: Nystatin-perforated patch-clamp recording;    Diethylstilbestrol;    Environmental endocrine disrupter;   

DOI  :  10.1254/jjp.84.225

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(2)The effect of a synthetic estrogen, diethylstilbestrol(DES), on kainate−induced currents was investigated in the hippocampal CA1 pyramidal neurons acutely dissociated from the mice using the nystatin−perforated patch−clamp recording configuration under voltage−clamp conditions.DES inhibited the current evoked by 100μM kainate in a concentration−dependent manner with a half−maximum inhibitory concentration of 8.8μM.The action of DES was voltage−independent.Since DES produced a suppression of the maximum response of the kainate concentration−response curve, the inhibition by DES of the kainate−induced current appears to be non−competitive.

【 授权许可】

Unknown