Immunohistochemical Demonstration of Cl- Pump in Type A Intercalated Cells of Rat Kidney

The Japanese Journal of Pharmacology

Sei Sasaki²  Fujio Shimizu³  Toshiaki Higashida⁴  Koichiro Omori¹  Mitsuyoshi Hara⁴  Michiaki Orikasa³  Xun-Ting Zeng⁴  Chiyoko Inagaki⁴ 

[1] Department of Physiology,Kansai Medical University,Moriguchi,Osaka 570-8506,Japan;Second Internal Medicine,Tokyo Medical and Dental University,Tokyo 113-8519,Japan;Department of Cell Biology,Institute of Nephrology,Niigata University School of Medicine,Niigata 951-8510,Japan;Department of Pharmacology,Kansai Medical University,Moriguchi,Osaka 570-8506,Japan

关键词: Cl- pump;    Ethacrynic acid;    Intercalated cell;    Collecting duct;    Kidney;   

DOI  :  10.1254/jjp.83.233

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(29)Cited-By(1)In order to demonstrate the localization of an ethacrynic acid−sensitive Cl- pump in the rat kidney, immunohistochemical analysis was performed using an anti−Cl- pump antibody raised against rat brain Cl- pump protein with confocal laser scanning microscopy.The antibodies against Na+, K+−ATPase, aquaporin 2 and a type B intercalated cell marker, 43−kDa protein, were also used for comparison.Anti−Cl- pump antibody recognized a 51−kDa renal protein of the same size as that in the brain on Western blots.Cl-−pump−like immunoreactivity was observed on the basolateral membranes of 42±3% of cortical collecting duct(CCD)cells and of 38±1% of outer medullar collecting duct(OMCD)cells.Such immunoreactivity in CCD was sometimes co−localized with Na+, K+−ATPase, but in OMCD, the Cl- pump−like immunoreactivity co−existed with neither Na+, K+−ATPase, aquaporin 2 nor the type B intercalated cell marker 43−kDa protein.Thus, the Cl- pump was demonstrated to be localized on the basolateral membranes of type A intercalated cells of cortical and medullary collecting ducts in the rat kidney.

【 授权许可】

Unknown