Endogenous ATP Released by Electrical Field Stimulation Causes Contraction via P2x- and P2y-Purinoceptors in the Isolated Tail Artery of Rats

The Japanese Journal of Pharmacology

Ai Fukumitsu¹  Takeshi Katsuragi²  Kenji Honda¹  Hiro-o Kamiya¹  Ayako Iki¹  Ryo Saito¹  Yukio Takano¹ 

[1] Department of Pharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University;Department of Pharmacology, School of Medicine, Fukuoka University

关键词: P2x-receptor;    P2y-receptor;    Tail artery;    ATP;    Vasocontraction;   

DOI  :  10.1254/jjp.81.375

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(30)Cited-By(4)Electrical field stimulation (EFS) caused contraction of isolated tail arteries of rats. The EFS-induced contraction showed frequency-dependence and was entirely abolished by the sodium channel blocker tetrodotoxin (1×10-7 M). The EFS-induced (at 20 Hz) contraction was reduced by about 60% in the presence of phentolamine (1×10-6 M). Therefore, later experiments were carried out in the presence of phentolamine. Pyridoxal-phosphate-6-azophenyl-2′, 4′-disulphonic acid (PPADS) (1×10-8 - 1×10-6 M) and basilen blue E-3G (3×10-5 - 5×10-5 M), P2-receptor antagonists, significantly inhibited the contraction evoked by EFS. In addition, PPADS significantly inhibited the contractions induced by ATP (1×10-4 M) and a selective P2x-receptor agonist, α, β-methylene ATP (1×10-6 M). In contrast, basilen blue E-3G did not inhibit α, β-methylene ATP-induced contraction. The ecto-ATPase activator apyrase (5 and 10 U/ml) significantly reduced the EFS-induced contractions. These findings suggest that endogenous ATP released by EFS causes contractions of rat tail artery via both the P2x-receptors and P2y-receptors.

【 授权许可】

Unknown