期刊论文详细信息
The Japanese Journal of Pharmacology
Protective Effect of Histidine on Hydroxyl Radical Generation Induced by Potassium-Depolarization in Rat Myocardium
Toshio Obata1  Masahiro Aomine2  Yasumitsu Yamanaka1 
[1] Department of Pharmacology, Oita Medical University;Division of Nutritional Physiology, Graduate School of Nutrition Science, Nakamura Gakuen University
关键词: Depolarization;    Potassium chloride;    Histidine;    Hydroxyl radical;    Microdialysis;   
DOI  :  10.1254/jjp.80.217
学科分类:药理学
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society
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【 摘 要 】

References(36)We investigated the efficacy of histidine on potassium-depolarization induced hydroxyl radical (·OH) generation in the extracellular fluid of rat myocardium by a flexibly mounted microdialysis technique (O system). After the rat was anesthetized, a microdialysis probe was implanted in the left ventricular myocardium, and then sodium salicylate in Ringer’s solution (0.5 nmol/μl per minute) was infused to detect the generation of ·OH as reflected by the nonenzymatic formation of 2, 3-dihydroxybenzoic acid (DHBA). Infusion of KCl (70 mM) clearly produced an increase in ·OH formation. However, when KCl in the presence of a high concentration of histidine (25 mM) was infused through the microdialysis probe, KCl failed to increase the 2, 3-DHBA formation. To examine the effect of histidine on ischemia-reperfusion of the myocardium, the heart was subjected to myocardial ischemia for 15 min by occlusion of the left anterior descending coronary artery (LAD). When the heart was reperfused, a marked elevation of the levels of 2, 3-DHBA was observed in the heart dialysate. However, when corresponding experiments were performed with histidine (25 mM)-pretreated animals, histidine prevented the ischemia-reperfusion induced ·OH formation trapped as 2, 3-DHBA. These results indicate that histidine may protect against K+-depolarization-evoked ·OH generation in rat myocardium.

【 授权许可】

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