The Japanese Journal of Pharmacology | |
Protective Effect of Histidine on Hydroxyl Radical Generation Induced by Potassium-Depolarization in Rat Myocardium | |
Toshio Obata1  Masahiro Aomine2  Yasumitsu Yamanaka1  | |
[1] Department of Pharmacology, Oita Medical University;Division of Nutritional Physiology, Graduate School of Nutrition Science, Nakamura Gakuen University | |
关键词: Depolarization; Potassium chloride; Histidine; Hydroxyl radical; Microdialysis; | |
DOI : 10.1254/jjp.80.217 | |
学科分类:药理学 | |
来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society | |
【 摘 要 】
References(36)We investigated the efficacy of histidine on potassium-depolarization induced hydroxyl radical (·OH) generation in the extracellular fluid of rat myocardium by a flexibly mounted microdialysis technique (O system). After the rat was anesthetized, a microdialysis probe was implanted in the left ventricular myocardium, and then sodium salicylate in Ringer’s solution (0.5 nmol/μl per minute) was infused to detect the generation of ·OH as reflected by the nonenzymatic formation of 2, 3-dihydroxybenzoic acid (DHBA). Infusion of KCl (70 mM) clearly produced an increase in ·OH formation. However, when KCl in the presence of a high concentration of histidine (25 mM) was infused through the microdialysis probe, KCl failed to increase the 2, 3-DHBA formation. To examine the effect of histidine on ischemia-reperfusion of the myocardium, the heart was subjected to myocardial ischemia for 15 min by occlusion of the left anterior descending coronary artery (LAD). When the heart was reperfused, a marked elevation of the levels of 2, 3-DHBA was observed in the heart dialysate. However, when corresponding experiments were performed with histidine (25 mM)-pretreated animals, histidine prevented the ischemia-reperfusion induced ·OH formation trapped as 2, 3-DHBA. These results indicate that histidine may protect against K+-depolarization-evoked ·OH generation in rat myocardium.
【 授权许可】
Unknown
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