Analgesia-Producing Mechanism of Processed Aconiti Tuber: Role of Dynorphin, an Endogenous κ-Opioid Ligand, in the Rodent Spinal Cord

The Japanese Journal of Pharmacology

Yasuhiro Komatsu¹  Yasuyuki Suzuki¹  Kazuhiro Goto¹  Atsushi Ishige¹  Yuji Omiya¹ 

[1] Kampo and Pharmacognosy Laboratories, Tsumura & Co.

关键词: Processed Aconiti tuber;    Antinociception;    Dynorphin;    Spinal cord;    Hypothalamic arcuate nucleus;   

DOI  :  10.1254/jjp.79.295

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(19)Cited-By(29)The analgesia-producing mechanism of processed Aconiti tuber was examined using rodents whose nociceptive threshold was decreased by loading repeated cold stress (RCS). The antinociceptive effect of processed Aconiti tuber (0.3 g/kg, p.o.) in RCS-loaded mice was antagonized by pretreatment with a κ-opioid antagonist, nor-binaltorphimine (10 mg/kg, s.c.), and was abolished by an intrathecal injection of anti-dynorphin antiserum (5 μg). The Aconiti tuber-induced antinociception was inhibited by both dexamethasone (0.4 mg/kg, i.p.) and a dopamine D2 antagonist, sulpiride (10 mg/kg, i.p.), in RCS-loaded mice, and it was eliminated by both an electric lesion of the hypothalamic arcuate nucleus (HARN) and a highly selective dopamine D2 antagonist, eticlopride (0.05 μg), administered into the HARN in RCS-loaded rats. These results suggest that the analgesic effect of processed Aconiti tuber was produced via the stimulation of κ-opioid receptors by dynorphin released in the spinal cord. It was also shown that dopamine D2 receptors in the HARN were involved in the expression of the analgesic activity of processed Aconiti tuber.

【 授权许可】

Unknown