Expression of Cytokine mRNA in Extrahepatic Organs in a Mouse Concanavalin A-Hepatitis Model

The Japanese Journal of Pharmacology

Tadashi Kobayashi²  Toshihiro Okamoto²  Yoshihisa Nakano²  Wataru Asakura²  Kaoru Hara²  Naoki Tsuzuike¹ 

[1] Zeria Pharmaceutical Co., Ltd.;Research Laboratories, Nippon Chemiphar Co., Ltd.

关键词: Concanavalin A;    Cytokine mRNA;    Extrahepatic organ;    Hepatitis;    Polymerase chain reaction;   

DOI  :  10.1254/jjp.77.219

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(16)Cited-By(6)The liver injury in the concanavalin A (Con A)-induced mouse hepatitis model has been well studied. However, there has been little study on the effects of Con A on extrahepatic organs. The aim of the present work was to determine the effects of Con A on the spleen, kidney and lung. A histopathological study showed that Con A (15 mg/kg, i.v.) administration affects not only the liver, but also all these extrahepatic organs. Messenger RNA expression was studied by the using polymerase chain reaction. Treatment with Con A induced interleukin-2 mRNA in the spleen, but only slightly induced it in the kidney. The mRNAs of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were induced in all these organs. At 24 hr after Con A treatment, the expression of IFN-γ mRNA, but not that of TNF-α mRNA, was inhibited by cyclosporine A (50 mg/kg, i.p.), suggesting that Con A induced these cytokine mRNAs through different mechanisms. In the kidney and lung, CD4+ and CD8+ T-cell infiltration was suggested by the Con A-induced CD4 and CD8 mRNAs. The present study showed the histopathological effects of Con A and Con A-induced cytokine mRNA expression on the spleen, kidney and lung.

【 授权许可】

Unknown