Effects of SS320A, a New Cysteine Derivative, on the Change in the Number of Goblet Cells Induced by Isoproterenol in Rat Tracheal Epithelium

The Japanese Journal of Pharmacology

Hirofumi Kai³  Hiromitsu Ohno¹  Koichi Takahashi¹  Kazuo Takahama³  Shigeru Nagaoka²  Hiroyuki Mizuno¹  Yoichiro Isohama³  Takeshi Miyata³ 

[1] Central Research Laboratories, SS Pharmaceutical Co., Ltd.;Japan Research Institute of Sputum;Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University

关键词: Cysteine derivative;    Goblet cell;    Isoproterenol;    SS320A;   

DOI  :  10.1254/jjp.77.71

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(28)Cited-By(10)We examined the effects of SS320A ((-)-(R)-2-amino-3-(3-hydroxypropylthio)propionic acid), a new cysteine derivative, on the change in the number of goblet cells induced by isoproterenol in rat tracheal epithelium. Four types of goblet cells were characterized in tracheal epithelium according to their size and staining affinity with Alcian blue (AB) / periodic acid Schiff (PAS). When each rat was given a single daily injection of isoproterenol (0.05 mg/kg, i.p.) for 14 days, a significant increase was observed in AB/PAS-positive cells that were recognizable as goblet cells in tracheal epithelium. When SS320A (10 - 100 mg/kg, p.o.) or propranolol (1 mg/kg, s.c.) was administered before each injection of isoproterenol, the increase in the number of goblet cells induced by isoproterenol was significantly inhibited. There was no difference between male and female rats with regard to this inhibitory action. On the other hand, ambroxol, bromhexine, L-cysteine ethyl ester and S-carboxymethylcysteine (100 mg/kg, p.o., respectively), which are used as expectorants, had no inhibitory effects on the isoproterenol-induced change in the number of goblet cells. Four metabolites (M1 - M4) of SS320A in rats also failed to inhibit the change induced by isoproterenol. These data suggest that SS320A itself may have a beneficial effect against mucus hypersecretion in chronic respiratory diseases.

【 授权许可】

Unknown